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Molecular Therapeutics: 21st Century Medicine

ISBN: 978-0-470-01916-0
264 pages
December 2007
Molecular Therapeutics: 21st Century Medicine (0470019166) cover image
"This book should be useful to lecturers who teach senior undergraduates, graduate students, and students in the biomedical sciences in general.  More globally, Greenwell and McCulley's book should encourage academicians of any stripe who for some time have been honing their lectures in a niche subject area to turn their courses into textbooks." –Biochemistry and Molecular Biology Education

Molecular therapeutics refers to the developments in molecular biology that are focused on treating disease with new molecular-based drugs.

By taking advantage of recent advances and increased understanding in the field of human genetics, this book provides essential background knowledge and key literature on a broad range of novel approaches and disciplines. These include making recombinant proteins, xenotransplantation gene therapy and therapeutic cloning. Molecular Therapeutics: 21st Century Medicine describes the techniques - including their associated benefits, problems, pitfalls - and discusses their applicability with respect to treating microbial, inherited, multifactoral and acquired diseases. The book also pays specific attention to the ethical issues associated with this new field.

  • Cutting-edge topics and clinically relevant materials engage and maintain student interest
  • Self-assessment questions are included throughout the book
  • Features an additional web site, with a web forum, regular updates and PowerPoint slides of figures from the book

Molecular Therapeutics: 21st Century Medicine is a comprehensive, accessible and engaging guide to the rapidly developing field of molecular therapeutics. It is essential reading for all students in this area of research and also of interest for health professionals involved in these novel therapies.

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Prologue.

1 Introduction.
1.1 Microbial diseases.
1.2 Cancer and heart disease.
1.3 Genetic diseases.
1.4 Role of molecular biology in therapeutics.

2 Prenatal diagnosis and pre-implementation.

2.1 Should we treat inherited diseases?
2.2 Genetic screening.
2.2.1 Pre-implementation genetic diagnosis.
2.3 Counselling.

3 Simple protein replacement therapy.
3.1 Preventing transfusion-transmissible infectious diseases in the UK.
3.2 Ensuring the safety of organ transplants.
3.3 Preventing transfusion-transmissible infectious diseases worldwide.
3.4 HIV.

4 Recombinant protein production.

4.1 Choice of organism.
4.2 Alternatives to E. coli for the production of recombinant proteins.
4.3 Problems with recombinant protein production.
4.4 All recombinants must be tested before they are given to humans.
4.5 Why make recombinant proteins?
4.6 Recombinant products.
4.7 Generics.

5 Recombinant vaccines.

5.1 Vaccine history.
5.2 Vaccines.
5.3 Vaccine methods.
5.4 Types of vaccine.
5.5 The limitations of vaccine programmes.
5.6 The role of the WHO.
5.7 Problems specific to developing countries.
5.8 Economics and logistics of vaccinology.
5.9 Recombinant vaccines.
5.10 Rational design: bioinformatics and proteomics.
5.11 Other interesting areas for vaccine development.
5.12 Conclusion.

6 Therapeutic antibodies and immunotherapy.
6.1 Monoclonal antibodies.
6.2 Monoclonal production.
6.3 Therapeutic monoclonal antibodies.
6.4 Transgenic monoclonals.
6.5 The uses of monoclonal antibodies in therapy.
6.6 Specific examples of therapeutic strategies.
6.7 Other recombinant proteins used in immunotherapy.

7 Transgenic animals.
7.1 Why do we want to engineer the genomes of animals?
7.2 Experimental procedure.
7.3 DNA constructs, insertional mutagenesis and homologous recombination.
7.4 Uses of inducible and tissue-specific promoters.
7.5 Introduction of the DNA into the cells.
7.6 Uses of transgenics.

8 Transplantation: a form of gene therapy.
8.1 Introduction.
8.2 Bone marrow.
8.3 Solid organ transplantation.
8.4 Other cells and tissues.
8.5 Summary of the problems associated with transplantation.
8.6 Transplantation statistics.
8.7 Legislation.
8.8 Religious beliefs and transplantation.

9 Xenotransplantation.

9.1 Introduction.
9.2 Rationale for the use of non-human donors.
9.3 Organs from non-human primates.
9.4 Pigs.
9.5 Problems with pigs.
9.6 Government legislation.
9.7 When will xenotransplantation start?
9.8 Patient attitudes.
9.9 Ethics.
9.10 Alternatives to xenotransplants.

10 Reproductive cloning

10.1 History.
10.2 Problems.
10.3 Why was there so much interest in Dolly?
10.4 Was Dolly a lone example?
10.5 Why is cloning useful?
10.6 Is human cloning a reality?
10.7 Why can we not produce human clones that are identical?
10.8 So why clone humans?
10.9 What are the ethical and moral problems?

11 Stem cell therapy.

11.1 The potency of cells.
11.2 Cloning.
11.3 Potency of stem cells.
11.4 Potential sources of stem cells.
11.5 Stem cells and therapeutic cloning.
11.6 Legislation and therapeutic cloning.
11.7 Other sources of stem cells.
11.8 What can be done?
11.9 Experiments on embryonic cells.
11.10 Experiments on fetal tissue and cord blood.
11.11 Stem cells from adult tissues.
11.12 Safety and technical problems.
11.13 Perceived scope of therapy.
11.14 Clinical trials of stem cell therapy.
11.15 What are the future prospects for stem cell research?

12 Gene augmentation therapy.

12.1 Introduction.
12.2 Strategy.

13 Gene therapy trials for inherited diseases.

13.1 Introduction.
13.2 Examples of disease treated with retroviral gene therapy.
13.3 Cystic fibrosis.
13.4 Animal trials with Factor IX.
13.5 Adenoviruses have also been used to introduce genes into brain.
13.6 Duchenne's muscular dystrophy.
13.7 Problems with adenoviruses.
13.8 The uses of adeno-associated viruses.
13.9 Liposome vector trials.
13.10 Trials with polymer mareix delivery.

14 Gene silencing technologies.

14.1 Antisense therapy.
14.2 Triple helix (triplex) technology.
14.3 Ribozymes.
14.4 Small interfering RNAs (siRNAs).

15 Gene therapy for cancer.

15.1 What causes cancer?
15.2 Cancer: a multifactorial disease.
15.3 Cancer statistics.
15.4 Best treatment currently available.
15.5 Do chemo- and radiotherapy cause problems?
15.6 New cancer therapies.
15.7 Cancer models in animals.
15.8 What kinds of gene therapy can we use to treat cancer?
15.9 Perceived problems in cancer gene augmentation therapy.
15.10 Gene silencing technologies and cancer.
15.11 Conclusion.

16 Single-nucleotide polymorphisms (SNPs) and therapy.

17 Legislation, clinical trials and ethical issues.
17 Legislation, clinical trials and ethical issues.
17.1 Legislative bodies.
17.2 Clinical trials.
17.3 The problems of placebo controlled trials.
17.4 The need for informed consent.
17.5 Trials in developing countries.
17.6 Recent trial issues.
17.7 Conclusion.

Epilogue.
Sourcing references.
Index.

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Pamela Greenwell is Principal Lecturer at The University of Westminster. She leads a research team in glycobiology, molecular biology and bioinformatics and is actively involved with enabling research in clinical trials between academics, industry and Primary Care Trusts.

Michelle McCulley has a background in human genetics and experience teaching a broad range of students and health professionals, she is currently a Senior Teaching fellow at the Peninsula Medical School.

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  • An accessible, engaging guide to molecular therapeutics for students
  • Cutting-edge topics and clinically relevant materials engage and maintain student interest.
  • Self-assessment questions are included throughout the book
  • Features an additional web site, with a web forum, regular updates and PowerPoint slides of figures from the book.
See More
Students Resources
Wiley Student Companion Site
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