Introducing Proteomics: From concepts to sample separation, mass spectrometry and data analysis
This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study.
The text covers the following:
- Separation Technologies
- Analysis of Peptides/Proteins by Mass Spectrometry
- Strategies in Proteomics
This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student.
This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems.
"I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues."
—Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin
1.1 What are the Tasks in Proteomics?
1.2 Challenges in Proteomics.
1.3 Proteomics in Relation to Other -omics and System Biology.
1.4 Some General Applications of Proteomics.
1.5 Structure of the Book.
2 Separation and Detection Technologies.
2.1 Introduction to Experimental Strategies in Proteomics.
2.2 Gel-Based Separation.
2.3 Visualization and Analysis of Proteins/Peptides in Gels.
2.4 Gel-Free Separation Technologies.
2.5. Visualization of Proteins/Peptides from Hyphenated Methods.
2.6 Chips in Proteomics Applications.
3 Analysis of Peptides/Proteins by Mass Spectrometry.
3.1 Basic Principles of Mass Spectrometry for Proteomics.
3.2 Ionization Methods for Small Amounts of Biomolecules.
3.3 Mass Aanalyzers and Mass Spectrometers.
3.4 Concluding Remarks on Mass Analyzers for Proteomics.
4 Analysis and Interpretation of Mass Spectrometric and Proteomic Data.
4.2 Analysis of MS Data.
4.3 Analysis of MS/MS Data.
4.4 Quantification of LC MS and MS/MS Data from Complex Samples.
4.5 Bioinformatic Approaches for Mass Spectrometric Proteome Data Analysis.
5 Strategies in Proteomics.
5.1 Imaging Mass Spectrometry.
5.2 Qualitative Proteomics.
5.3 Differential and Quantitative Proteomics.
5.4 Analysis of Posttranslational Modifications.
5.6 Proteomics as Part of Integrated Approaches.