Current Protocols Essential Laboratory TechniquesISBN: 978-0-470-08993-4
Paperback
806 pages
February 2008
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Preface.
Contributors.
Common Conversion Factors.
Combining Techniques to Answer Molecular Questions.
Chapter 1: Volume/Weight Measurement.
1.1 Volume Measurement.
1.2 Weight Measurement.
Chapter 2: Concentration Measurement.
2.1 Spectrophotometry.
2.2 Quantitation of Nucleic Acids and Proteins.
2.3 Radiation Safety and Measurement.
Chapter 3: Reagent Preparation.
3.1 Reagent Preparation: Theoretical and Practical Discussions.
3.2 Measurement of pH.
3.3 Recipes for Commonly Encountered Reagents.
Chapter 4: Cell Culture Techniques.
4.1 Aseptic Technique.
4.2 Culture of Escherichia coli and Related Bacteria.
Chapter 5: Sample Preparation.
5.1 Centrifugation.
5.2 Purification and Concentration of Nucleic Acids.
Chapter 6: Chromatography.
6.1 Overview of Chromatography.
6.2 Column Chromatography.
Chapter 7: Electrophoresis.
7.1 Overview of Electrophoresis.
7.2 Agarose Gel Electrophoresis.
7.3 SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
7.4 Staining Proteins in Gels.
7.5 Overview of Digital Electrophoresis Analysis.
Chapter 8: Blotting.
8.1 Overview of Blotting.
8.2 Nucleic Acid Blotting: Southern and Northern.
8.3 Protein Blotting: Immunoblotting.
8.4 Labeling DNA and Preparing Probes.
Chapter 9: Microscopy.
9.1 Conventional Light Microscopy.
9.2 Immunofluorescence Microscopy.
Chapter 10: Enzymatic Reactions.
10.1 Working with Enzymes.
10.2 Overview of PCR.
10.3 Real-Time PCR.
10.4 DNA Sequencing: An Outsourcing Guide.
Appendices.
A.1 Laboratory Safety.
A.2 Laboratory Notebooks and Data Storage.
A.3A Ethical Considerations When Altering Digital Images.
A.3B Practical Considerations When Altering Digital Images.
A.4 Statistical Analysis.
A.5A Preparing and Presenting a Poster.
A.5B Preparing and Presenting a Talk.
Index.
