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Current Protocols Essential Laboratory Techniques

Sean R. Gallagher (Editor), Emily A. Wiley (Editor)
ISBN: 978-0-470-08993-4
806 pages
February 2008
Current Protocols Essential Laboratory Techniques (0470089938) cover image
2008 PROSE award winner for Excellence in Biology and the Life Sciences

From the leading branded source for methods in the life sciences, this essential resource for the lab provides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world.

  • Developed by Current Protocols, the most trusted and recognized source of protocols
  • Teaches new investigators how to perform basic laboratory research techniques
  • Describes solution chemistry and preparation
  • Covers basic laboratory safety
  • Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes
  • Teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparation

This book is an invaluable resource for researchers in all areas of the life sciences, especially molecular biology, biotechnology, genetics, and immunology. It is essential reading for undergraduate and graduate students alike and is also of interest for investigators new to life science research.

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Foreword.

Preface.

Contributors.

Common Conversion Factors.

Combining Techniques to Answer Molecular Questions.

Chapter 1: Volume/Weight Measurement.

1.1 Volume Measurement.

1.2 Weight Measurement.

Chapter 2: Concentration Measurement.

2.1 Spectrophotometry.

2.2 Quantitation of Nucleic Acids and Proteins.

2.3 Radiation Safety and Measurement.

Chapter 3: Reagent Preparation.

3.1 Reagent Preparation: Theoretical and Practical Discussions.

3.2 Measurement of pH.

3.3 Recipes for Commonly Encountered Reagents.

Chapter 4: Cell Culture Techniques.

4.1 Aseptic Technique.

4.2 Culture of Escherichia coli and Related Bacteria.

Chapter 5: Sample Preparation.

5.1 Centrifugation.

5.2 Purification and Concentration of Nucleic Acids.

Chapter 6: Chromatography.

6.1 Overview of Chromatography.

6.2 Column Chromatography.

Chapter 7: Electrophoresis.

7.1 Overview of Electrophoresis.

7.2 Agarose Gel Electrophoresis.

7.3 SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE).

7.4 Staining Proteins in Gels.

7.5 Overview of Digital Electrophoresis Analysis.

Chapter 8: Blotting.

8.1 Overview of Blotting.

8.2 Nucleic Acid Blotting: Southern and Northern.

8.3 Protein Blotting: Immunoblotting.

8.4 Labeling DNA and Preparing Probes.

Chapter 9: Microscopy.

9.1 Conventional Light Microscopy.

9.2 Immunofluorescence Microscopy.

Chapter 10: Enzymatic Reactions.

10.1 Working with Enzymes.

10.2 Overview of PCR.

10.3 Real-Time PCR.

10.4 DNA Sequencing: An Outsourcing Guide.

Appendices.

A.1 Laboratory Safety.

A.2 Laboratory Notebooks and Data Storage.

A.3A Ethical Considerations When Altering Digital Images.

A.3B Practical Considerations When Altering Digital Images.

A.4 Statistical Analysis.

A.5A Preparing and Presenting a Poster.

A.5B Preparing and Presenting a Talk.

Index.

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  • Developed by Current Protocols, the most trusted and recognized source of protocols, this book is designed to be the "bible" of basic techniques.

  • In addition to providing the novice step-by-step procedures to perform basic techniques, it also provides the theory behind those techniques. In today's modern research lab, researchers use kits and the theory is being lost - a big problem noted by virtually every senior researcher.

  • This book will provide a clear, concise manual that will be an invaluable guide to the graduate student or anyone else coming into the life sciences laboratory. It covers most, if not all, of the techniques that life science researchers use on a daily basis, regardless of their education level.

  • The book is designed to be used as a textbook in upper-division undergraduate classes, where there is currently no standard text.

  • Much more up-to-date than anything currently available, the book covers digital imaging, photoshop, preparing posters from PowerPoint, and electronic notebooks - information that has not yet been published in any lab skills manual. It also covers real time-PCR, a very "hip" and extensively used technique that most books do not cover.

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