Table of Contents

Current Protocols Essential Laboratory Techniques (CPET) provides every researcher with the skills and understanding of fundamental laboratory procedures needed to ensure greater success at the bench. CPET takes the novice researcher from very basic skills like weight and volume measurement, through reagent preparation and the use of routine instrumentation, and finally into advanced topics such as real-time PCR and bioinformatics. In addition CPET ...

• Teaches new investigators how to formulate basic research questions and plan the experiments needed to answer them
• Describes solution chemistry and preparation
• Covers basic laboratory safety
• Provides instruction on the care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes
• Teaches how to manage information from lab notebooks, images, and literature references, as well as manuscript preparation
• Details modern bioinformatics techniques
• Provides practical guides to outsourcing tasks such as sequencing and oligonucleotide synthesis

Edited by: Sean R. Gallagher (UVP, Inc.) and Emily A. Wiley (Claremont Colleges); Guest Editors: Andre Ricardo de Oliveira Cavalcanti (Pomona College) and Nicholas A. Stover (Bradley University)

Developmental Editor: Tom Downey

While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.