What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice, 2nd Edition
Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters:
"Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis.
"Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.
The final chapter, "The Next Generation," examines new and emerging technologies.
Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.
Preface to the First Edition.
Chapter 1. Designer Mice.
Chapter 2. Of Unicorns and Chimeras.
Chapter 3. General Health.
Chapter 4. Motor Functions.
Chapter 5. Sensory Abilities.
Chapter 6. Learning and Memory.
Chapter 7. Feeding and Drinking.
Chapter 8. Reproductive Behaviors.
Chapter 9. Social Behaviors.
Chapter 10. Emotional Behaviors: Animal Models of Psychiatric Diseases.
Chapter 11. Reward.
Chapter 12. Neurodevelopment and Neurodegeneration
Chapter 13. Putting It All Together.
Chapter 14. The Next Generation.
What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice, Second Edition has been written in Dr. Crawley's private capacity, outside of her professional position at the National Institutes of Health. The views expressed in this book do not necessarily represent the views of the National Institutes of Health or of the United States government.
Answers the questions: What are the best tests? What controls should be used? How do you avoid potential hidden pitfalls, false positives and negatives, and lurking artifacts? Which statistical tests can be applied?
Includes a new chapter on Developmental and Neurodegenerative Diseases, covering mental retardation and inborn errors of metabolism through to Alzheimer's disease and stroke
Provides brand new coverage of practical recommendations for choosing tasks to evaluate a new mutant line (Putting It All Together), including complete guidelines for high-throughput screening
Discusses implications of the completion of the mouse genome sequence, microarray technology, new gene delivery methods, stem cell transplantation, proteomics, and high throughput approaches in chemical mutagenesis studies
Expands discussion to the latest studies in learning and memory, autism and social phobias, olfaction, drug addiction, withdrawal, and spinal cord injury
Includes numerous new figures showing new behavioral tests, equipment, and data
Each chapter concludes with extensive, up-to-date references and suggestions for background reading
"The book successfully brings together the field of behavioral neuroscience and the rewards and insights that can be gained using this approach. I would recommend that every behavioral scientist has at least two copies, one for their own use and one that will be on permanent load to their students, post-doctoral students and colleagues in molecular biology." (Trends in Pharmaceutical Sciences)
"the overall impression is of a helpful guide taking the reader step by step through the complexities of animal behavioral research." (Nature Neuroscience)
"succeeds as a useful introduction to the realm of behavioral phenotyping for those interested in creating or using the large and increasing number of promising targeted mutant mice." (Trends in Neurosciences)