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Primary and Stem Cells: Gene Transfer Technologies and Applications

ISBN: 978-0-470-61074-9
304 pages
November 2011
Primary and Stem Cells: Gene Transfer Technologies and Applications (0470610743) cover image
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
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Michele Calos




1. DNA Assembly Technologies Based on Homologous Recombination 3
Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen

2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells 19
Takefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan D. Chesnut

3. Strategies for the Delivery of Naked DNA 37
Jennifer C. Moore and Ronald P. Hart


4. Episomal Vectors 51
Junying Yu

5. Nonintegrating DNA Virus 85
Chris Kemp and David Kuninger

6. Nonintegrating RNA Viruses 103
Noemi Fusaki

7. Protein Delivery 119
Xiao-Jian Han and Kazuhito Tomizawa


8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery 137
Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and R. Scott McIvor

9. Integrating Viral Vectors for Gene Modifications 163
Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek

10. Bacteriophage Integrases for Site-Specific Integration 199
Bhaskar Thyagarajan

11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211
Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu


12. Modified Stem Cells as Disease Models and in Toxicology Screening 229
Catharina Ellerstr€om, Peter Sartipy, Raimund Strehl, and Johan Hyllner

13. Screening and Drug Discovery 251
Elizabeth A. Davenport and Robert S. Ames


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UMA LAKSHMIPATHY is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications.

BHASKAR THYAGARAJAN is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.

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“This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells.  Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy.”  (Doody’s, 17 August 2012)




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Primary and Stem Cells: Gene Transfer Technologies and Applications (US $119.00)

-and- Environmental Biotechnology: Theory and Application, 2nd Edition (US $92.95)

Total List Price: US $211.95
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