Advanced Techniques in Diagnostic Cellular Pathology

Preface.

List of Tables.

List of Figures.

Supplementary Materials.

1 Virtual Microscopy (Jim Diamond and David McCleary).

1.1 Introduction.

1.2 Digital (virtual) microscopy: equipment for implementation.

1.3 The virtual slide format.

1.4 Image serving and viewing.

1.5 Applications of virtual microscopy.

1.6 Virtual microscopy in education.

1.7 Computational aspects of virtual microscopy.

1.8 Conclusions.

2 Cytopathology (Mary Hannon-Fletcher).

2.1 Introduction.

2.2 Basic principles.

2.3 Cytodiagnosis.

2.4 Gynaecological cytopathology.

2.5 Conclusions.

3 Flow Cytometry (Ian Dimmick).

3.1 Introduction.

3.2 Sample preparation.

3.3 Principles of the flow cytometer.

3.4 Clinical applications.

3.5 The future for flow cytometry.

3.6 Conclusions.

4 Immunocytochemistry (Perry Maxwell and Merdol Ibrahim).

4.1 Introduction.

4.2 Basic principles.

4.3 Clinical immunocytochemistry.

4.4 Conclusions.

5 Microarray-based Comparative Genomic Hybridization (David S.P. Tan, Rachael Natrajan and Jorge S. Reis-Filho).

5.1 Introduction.

5.2 Principles of array CGH.

5.3 aCGH platforms.

5.4 Choosing the right platform.

5.5 Analysis and validation.

5.6 Finding the target.

5.7 Data integration.

5.8 Clinical applications.

5.9 Conclusions.

6 Tissue In Situ Hybridization (Anthony O’ Grady, John O’ Loughlin and Hilary Magee).

6.1 Introduction.

6.2 ISH probes.

6.3 Probe labels.

6.4 ISH detection systems.

6.5 Tissue preparation and the ISH procedure.

6.6 Signal amplification.

6.7 ISH controls.

6.8 Clinical applications of tissue ISH.

6.9 ISH automation.

6.10 Image capture and analysis.

6.11 Recent developments and future directions in tissue ISH.

6.12 Conclusions.

Index.