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Current Protocols in Essential Laboratory Techniques

ISBN: 978-0-470-94241-3
Approx. 800 pages
Current Protocols in Essential Laboratory Techniques (047094241X) cover image

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What's New in Current Protocols Essential Laboratory Techniques
Supplement 8, February 2014

Unit 6.3 Thin Layer Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 7.1 Overview of Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 10.3 Real-Time PCR
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Using NCBI BLAST
         Abstract | Full Text:  HTML   PDF

Suitable for novice and expert alike, Current Protocols Essential Laboratory Techniques is the ultimate all-in-one fundamentals guide for life scientists. It provides in-depth information for experienced researchers looking for fresh insight into fundamental techniques, while remaining accessible to young scientists preparing to run an electrophoresis gel for the first time.

A subscription gives you access to all the content in the collection plus one year of issues of new and updated content. Current Protocols Essential Laboratory Techniques

  • teaches new investigators how to formulate basic research questions and plan the experiments needed to answer them
  • provides instruction on the care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes
  • teaches how to manage information from lab notebooks, images, and literature references, as well as manuscript preparation
  • describes solution chemistry and preparation
  • covers basic laboratory safety
  • provides practical guides to outsourcing tasks such as sequencing and oligonucleotide synthesis

Edited by: Sean R. Gallagher (UVP, LLC) and Emily A. Wiley (Claremont McKenna, Pitzer, and Scripps Colleges); Guest Editors: Andre Ricardo de Oliveira Cavalcanti (Pomona College) and Nicholas A. Stover (Bradley University)

Developmental Editor: Viirgina Chanda

While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.

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Editors & Contributors


EDITORIAL BOARD

Sean R. Gallagher
UVP, LLC
Upland, California

Emily A. Wiley
Joint Science Department
Claremont, McKenna, Pitzer, and Scripps Colleges
Claremont, California

GUEST EDITORS

Andre R.O. Cavalcanti
Pomona College
Claremont, California

Nicholas A. Stover
Bradley University
Peoria, Illinois

CONTRIBUTORS

Jennifer A. Armstrong
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

David J. Asai
Harvey Mudd College
Claremont, California

Donna Bozyczko-Coyne
Cephalon, Inc.
Frazer, Pennsylvania

LouAnn C. Burnett
Vanderbilt University
Nashville, Tennessee

Steve Bursik
The Salk Institute
La Jolla, California

Tomasz Bykowski
University of Kentucky College of Medicine
Lexington, Kentucky

Li Cai
Division of Mathematics and Science
University of South Carolina Salkehatchie
Walterboro, South Carolina

Tuoxin Cao
Spinal Cord & Brain Injury Research Center
University of Kentucky College of Medicine
Lexington, Kentucky, and
Department of Anatomy & Neurobiology
University of Kentucky College of Medicine
Lexington, Kentucky

David Carter
Center for Plant Cell Biology
University of California at Riverside
Riverside, California

Andre R.O. Cavalcanti
Pomona College
Claremont, California

Bulbul Chakravarti
Proteomics Center
Keck Graduate Institute of Applied Life Sciences
Claremont, California

Deb N. Chakravarti
Proteomics Center
Keck Graduate Institute of Applied Life Sciences
Claremont, California

Stephen Chang
Keck Graduate Institute of Applied Life Sciences
Claremont, California

Wei-Jen Chang
Hamilton College
Clinton, New York

Thomas Chappell
Biogrammatics, Inc.
Carlsbad, California

John M. Christie
Institute of Molecular Cell and Systems Biology
College of Medical, Veterinary and Life Sciences
University of Glasgow
Glasgow, United Kingdom

Buena Chui
GE Healthcare
Piscataway, New Jersey

Eric S. Cole
St. Olaf College
Northfield, Minnesota

Thomas W. Coppola
Hamilton College
Clinton, New York

James M. Cregg
Keck Graduate Institute of Applied Life Sciences
Claremont, California and
Biogrammatics, Inc.
Carlsbad, California

Thomas Davis
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

Phillippe Desjardins
Thermo Scientific NanoDrop Products
Wilmington, Delaware

Leroi V. DeSouza
Centre for Research in Mass Spectrometry and Department of Chemistry
York University
Toronto, Ontario, Canada

Bruce Dorsey
Cephalon, Inc.
Frazer, Pennsylvania

Dennis H. Dowhan
University of Queensland
Brisbane, Queensland, Australia

Stacia R. Engel
Stanford University of Medicine
Stanford, California

Steven Fenster
Fort Lewis College
Durango, Colorado

Vincent A. Fischetti
Vincent A. Fischetti Laboratory of Bacterial Pathogenesis and Immunology
The Rockefeller University
New York, New York

Dean Fraga
College of Wooster
Wooster, Ohio

Deborah J. Frank
Washington University
St. Louis, Missouri

Sean R. Gallagher
UVP, LLC
Upland, California

Michael Guzy
Ohaus Corporation
Pine Brook, New Jersey

Matthew Hall
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

Johanna Hardin
Pomona College
Claremont, California

Karl A. Haushalter
Harvey Mudd College
Claremont, California

Laura L. Mays Hoopes
Pomona College
Claremont, California

Nick Huang
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

John Kloke
Pomona College
Claremont, California

Henry M. Krause
Banting and Best Department of Medical Research
University of Toronto
Toronto, Ontario, Canada and
The Donnelly Centre for Cellular and Biomolecular Research
University of Toronto
Toronto, Ontario, Canada

Anasua Kusari
Keck Graduate Institute of Applied Life Sciences
Claremont, California

Christine D. Kuslich
Molecular Profiling Institute
Phoenix, Arizona

Scott Larsen
Cephalon, Inc.
Frazer, Pennsylvania

Claudia Y. Lee
UVP, LLC
Upland, California

Jonathan Lifshitz
Spinal Cord & Brain Injury Research Center
University of Kentucky College of Medicine
Lexington, Kentucky,
Department of Anatomy & Neurobiology
University of Kentucky College of Medicine
Lexington, Kentucky, and
Department of Physical Medicine & Rehabilitation
University of Kentucky College of Medicine
Lexington, Kentucky

George Lunn
Baltimore, Maryland

Knut Madden
Biogrammatics, Inc.
Carlsbad, California

Buddhadeb Mallik
Proteomics Center
Keck Graduate Institute of Applied Life Sciences
Claremont, California

Glenn M. Manthey
City of Hope
Beckman Research Institute
Duarte, California

Stephen D. Mastrian
Translational Genomics Research Institute
Phoenix, Arizona

Scott Medberry
Agilent Technologies, Inc.
Palo Alto, California

Jill Meisenhelder
The Salk Institute
La Jolla, California

Tea Meulia
Ohio Agricultural Research and Development Center
Wooster, Ohio

Butch Moomaw
Hamamatsu Photonic Systems
Spring Branch, Texas

Rob Morris
Ocean Optics
Dunedin, Florida

Sreenivasa U.M. Murthy
Banting and Best Department of Medical Research
University of Toronto
Toronto, Ontario, Canada and
The Donnelly Centre for Cellular and Biomolecular Research
University of Toronto
Toronto, Ontario, Canada

Maria Cristina Negritto
Pomona College
Claremont, California

Benson Ngo
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

Ronald E. Pearlman
Centre for Research in Mass Spectrometry and Department of Biology
York University
Toronto, Ontario, Canada

Adam J. Pelzek
New York University School of Medicine
New York, New York

Sean P. Riley
University of Kentucky College of Medicine
Lexington, Kentucky

Daisuke Sakai
Nara Institute of Science and Technology
Nara, Japan

Lisa Sandell
Stowers Institute for Medical Research
Kansas City, Missouri

Cindy Santangelo
Worthington Biochemical
Lakewood, New Jersey

Joachim Sasse
Biomedtech Laboratories, Inc.
Tampa, Florida

Raymond Schuch
ContraFect Corporation
Yonkers, New York

Jonathan E. Schmitz
Vanderbilt University Medical Center
Nashville, Tennessee

Joseph R. Schulz
Occidental College
Los Angeles, California

Jerry Sedgewick
University of Minnesota
Minneapolis, Minnesota

Michael Siu
Centre for Research in Mass Spectrometry and Department of Chemistry
York University
Toronto, Ontario, Canada

Christine Sjolander
Keck Graduate Institute of Applied Life Sciences
Claremont, California

David Skrincosky
Worthington Biochemical
Lakewood, New Jersey

Kristin M. Slade
Joint Science Department
Claremont, McKenna, Pitzer, and Scripps College
Claremont, California

Brian Stevenson
University of Kentucky College of Medicine
Lexington, Kentucky

Nicholas A. Stover
Bradley University
Peoria, Illinois

Warren Strober
National Institute of Allergy and Infectious Diseases
Bethesda, Maryland

Anthony Jay Sunga
Keck Graduate Institute of Applied Life Sciences
Claremont, California

Pooja M. Talauliker
Spinal Cord & Brain Injury Research Center
University of Kentucky College of Medicine
Lexington, Kentucky, and
Department of Anatomy & Neurobiology
University of Kentucky College of Medicine
Lexington, Kentucky

Samuel E. Taylor
University of Bath
Bath, United Kingdom,
Spinal Cord & Brain Injury Research Center
University of Kentucky College of Medicine
Lexington, Kentucky, and
Department of Anatomy & Neurobiology
University of Kentucky College of Medicine
Lexington, Kentucky

Diane M. Thomson
Joint Science Department
Claremont, McKenna, Pitzer, and Scripps College
Claremont, California

Ilya Tolstorukov
Keck Graduate Institute of Applied Life Sciences
Claremont, California and
Biogrammatics, Inc.
Carlsbad, California

Sharon Torigoe
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

Jeffrey W. Touchman
Arizona State University
Tempe, Arizona

Jeffrey W. Touchman
Arizona State University
Tempe, Arizona

S. Vivek
Liberation Technology Program
Stanford University
Stanford, California

Ronit Wilk
Banting and Best Department of Medical Research
University of Toronto
Toronto, Ontario, Canada and
The Donnelly Centre for Cellular and Biomolecular Research
University of Toronto
Toronto, Ontario, Canada

Michael Williams
Cephalon, Inc.
Frazer, Pennsylvania

Michael E. Woodman
University of Kentucky College of Medicine
Lexington, Kentucky

Carl T. Yamashiro
Arizona State University
Tempe, Arizona

Haixu Yan
Banting and Best Department of Medical Research
University of Toronto
Toronto, Ontario, Canada and
The Donnelly Centre for Cellular and Biomolecular Research
University of Toronto
Toronto, Ontario, Canada

Andrew Zanella
Joint Science Department
Claremont McKenna, Pitzer, and Scripps Colleges
Claremont, California

Rebecca A. Zufall
University of Houston
Houston, Texas

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What's New


Supplement 8, February 2014


Unit 6.3 Thin Layer Chromatography
         Abstract | Full Text:  HTML   PDF

Unit 7.1 Overview of Electrophoresis
         Abstract | Full Text:  HTML   PDF

Unit 10.3 Real-Time PCR
         Abstract | Full Text:  HTML   PDF

Unit 11.1 Using NCBI BLAST
         Abstract | Full Text:  HTML   PDF

See More

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