Replacing Animal Models: A Practical Guide to Creating and Using Culture-based Biomimetic Alternatives

Preface, xi

Section 1 Introductory Material

1 Potential Advantages of Using Biomimetic Alternatives, 3
Jamie Davies

2 Overview of Biomimetic Alternatives, 13
Jamie Davies

Section 2 Culture Methods

3 Pancreatic Islets, 23
Eli C Lewis

4 Endometrial Organoid Culture, 35
Merja Blauer

5 Modelling Lymphatic and Blood Capillary Patterning, 45
Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel

6 Precision-cut Lung Slices (PCLS), 57
Christian Martin and Stefan Uhlig

7 Human Colon Tissue in Organ Culture, 69
Michael K Dame and James Varani

8 Fetal Organ Culture, 81
Jamie Davies

9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion, 89
Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein-Ritter

10 Magnetic Assembly of Tissue Surrogates, 107
Chien-Yu Fu and Hwan-You Chang

11 Assembly of Renal Tissues by Cellular Self-organization, 115
Mathieu Unbekandt

Section 3 Case Studies of Use

12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice, 125
Guangping Tai and Jamie Davies

13 Lung Organoid Culture to Study Responses to Viruses, 137
Wenxin Wu, J Leland Booth and Jordan P Metcalf

14 Organ-cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma, 151
James Varani

15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology, 159
Alastair J Sloan, Sarah Y Taylor and Emma L Smith

16 Three-dimensional, High-density and Tissue Engineered Culture Models of Articular Cartilage, 167
Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei

17 Concluding Remarks, 193
Jamie Davies

Appendix 1 Sources of funding for development of culture-based alternatives, 195

Appendix 2 Databases and web-based discussions relevant to development of alternatives, 197

Index, 199