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Chemical Measurements in Biological Systems

ISBN: 978-0-471-13903-4
280 pages
May 2000
Chemical Measurements in Biological Systems (0471139033) cover image
Techniques in Analytical Chemistry
How to evaluate and validate data from chemical measurements in biological systems-a unique overview
An understanding of chemical measurement processes in biological systems is essential to working in today's life-science and biotechnology industries. This work provides analysts and laboratory professionals with the fundamental knowledge and basic principles they need to tackle the measurement process in the real world. Rather than rehashing traditional analytical techniques, which can be problematic for biological samples, the book emphasizes the highly complex nature of biological systems through such topics as enzyme measurements, enzymes as reagents, immunochemical assays, and assay validation and quality control. It provides a complete overview of principles and methods proven useful in biological systems-from pH, buffers, and spectrophotometry to basic chromatographic techniques. With Chemical Measurements in Biological Systems as their guide, readers will understand the basic principles needed to:
* Read and evaluate an assay method description
* Choose the appropriate method from the vast array of available techniques
* Modify and evaluate an existing method for specific needs
* Set up and run biochemical assays
* Use multistep problem-solving techniques
* Evaluate the results of a given assay in a given matrix
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Water, pH, and Buffers.

Ultraviolet and Visible Absorption Spectrophotometry and Photometry.

Detection Reactions (Colorimetric Reactions).

Enzymes and the Use of Enzymes as Reagents.

Measurement of Enzyme Activities.

Chromatography as a Tool for Measurement of Analytes in Complex Mixtures.

Electrophoresis and Other Electrokinetic Separations.

Enzyme-Linked Immunosorbant Assays.

Assay Quality Control and Data Validation.

Method Selection.

Appendices.

Index.
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KENT K. STEWART, PhD, is Professor Emeritus of Biochemistry at Virginia Polytechnic Institute and State University, and Adjunct Professor of Chemistry and Biochemistry at the University of Texas at Austin. RICHARD E. EBEL, PhD, is Associate Professor in the Department of Biochemistry, Virginia Polytechnic Institute and State University.
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"...Stewart and Ebel offer a textbook for graduate or advanced undergraduate students in life sciences and a reference for researchers. They introduce the fundamentals of making chemical measurements..." (SciTech Book News, Vol. 24, No. 4, December 2000)

"In general, this is a book, which could be of enormous utility for people working in biochemistry." (The Analyst, 8th October, 2001)

"there was a lot here that I liked and I found myself nodding in agreement" (Chromatographia, November 2001)

"The style is readable and didactic and I think it succeeds as a basic primer." (Chromatographia)
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