Table of Contents

Individual Online Subscriptions are now available! Visit the How to Order page for more information.

What's New in Current Protocols in Cytometry
Supplement 45, July 2008

UNIT 4.7 Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries
UNIT 6.25 Flow Cytometric Immunophenotyping of Cerebrospinal Fluid
UNIT 7.34 Cell Proliferation Method: Click Chemistry Based on BrdU Coupling for Multiplex Antibody Staining
UNIT 12.13 Use of Spectral Fluorescence Resonance Energy Transfer to Detect Nitric Oxide-Based Signaling Events in Isolated Perfused Lung
UNIT 12.14 Flow Cytometric FRET Analysis of ErbB Receptor Tyrosine Kinase Interaction

Published in affiliation with the International Society for Analytical Cytology , Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.

Updated every three months in all formats, CPCY is constantly evolving to keep pace with the very latest discoveries and developments. A year of these quarterly updates is included in the initial CPCY purchase price. That's 470 pages of new (52%) and revised (48%) content on average every year since the initial publication of the work in October 1997! Presently two volumes in its looseleaf print version, CPCY...

• provides complete coverage of flow cytometry, with hundreds of basic to advanced techniques from instrumentation to interpretation; fluorescence to antigen density; research to clinical applications.
• offers in-depth coverage of microscopy, including detailed discussion of objectives and expert guidelines on image interpretation, confocal microscopy, and illumination sources.
• includes valuable data, plus descriptions of stock solutions, equipment, common techniques and more in three appendices.

Edited by: J. Paul Robinson, Managing Editor (Purdue University Cytometry Laboratories); Zbigniew Darzynkiewicz (New York Medical College); Robert Hoffman (BD Biosciences); John Nolan (La Jolla Bioengineering Institute); Alberto Orfao (Universidad de Salamanca); Peter Rabinovitch (University of Washington); Simon Watkins (University of Pittsburgh); Past Editors: Phillip N. Dean, Jurek Dobrucki, Lynn G. Dressler, Carleton C. Stewart, Hans J. Tanke, Leon L. Wheeless

Series Editor: Tom Downey

While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.