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HPLC Columns: Theory, Technology, and Practice

ISBN: 978-0-471-19037-0
416 pages
July 1997
HPLC Columns: Theory, Technology, and Practice (0471190373) cover image


An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include:
* Comprehensive overview of state-of-the-art HPLC column technology
* Explanation of the underlying principles of HPLC columns
* Methods for selecting columns
* Practical advice on using and applying columns, including examples
* Section by M. Zoubair El Fallah on methods development
* Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed
* Troubleshooting section
HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.
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Table of Contents

Theory of Chromatography.

Column Design.

Physical Properties of HPLC Packings.

Column Packing and Testing.

Column Chemistry.

Column Selection.

Size-Exclusion Chromatography.

Normal-Phase Chromatography.

Reversed-Phase Chromatography.

Hydrophilic Interaction Chromatography.

Ion-Exchange Chromatography.

Hydrophobic Interaction Chromatography.

Special Techniques.

Preparative Chromatography.

HPLC Methods Development.

Column Maintenance.


List of Symbols.

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Author Information

UWE D. NEUE, PhD, is a chemist in the Chemical Products Group at Waters Corporation, Milford, Massachusetts. Much of this book is based on seminars and lectures he conducted for colleagues and customers.
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