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Flow Cytometry: First Principles, 2nd Edition

ISBN: 978-0-471-38224-9
296 pages
July 2001
Flow Cytometry: First Principles, 2nd Edition (0471382248) cover image


Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.

Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:

  • Assumes no prior knowledge of flow cytometry
  • Progresses with an informal, engaging lecture style from simpleto more complex concepts
  • Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
  • Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields

Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

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Table of Contents


Acknowledgments (First Edition).

Acknowledgments (Second Edition).

The Past as Prologue.

Setting the Scene.

Instrumentation: Into the Black Box.

Information: Harnessing the Data.

Seeing the Light: Lasers, Fluorochromes, and Filters.

Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating.

Cells from Within: Intracellular Proteins.

Cells from Within: DNA in Life and Death.

The Sorting of Cells.

Disease and Diagnosis: The Clinical Laboratory.

Out of the Mainstream: Research Frontiers.

Flowing On: The Future.

General References.


Figure Credits.

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"...reflects innovations in the field since the first edition was published..." (SciTech Book News, Vol. 26, No. 2, June 2002)

"I consider it to be the best presentation of principles of flow cytometry. The text is clearly written, the figures are well chosen." —Zbigniew Darzynkiewicz, Brander Cancer Research Institute, New York Medical College

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