 Culture of Animal Cells: A Manual of Basic Technique, 5th Edition
ISBN: 978-0-471-45329-1
Hardcover
672 pages
July 2005
US $100.95
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List of Color Plates.
Preface.
Abbreviations.
1. Introduction.
2. Training Programs.
3. Biology of Cultured Cells.
4. Laboratory Design and Layout.
5. Equipment.
6. Aseptic Technique.
7. Safety, Bioethics, and Validation.
8. Culture Vessels and Substrates.
9. Defined Media and Supplements.
10. Serum-Free Media.
11. Preparation and Sterilization.
12. Primary Culture.
13. Subculture and Cell Lines.
14. Cloning and Selection.
15. Cell Separation.
16. Characterization.
17. Differentiation.
18. Transformation and Immortalization.
19. Contamination.
20. Cryopreservation.
21. Quantitation.
22. Cytotoxocity.
23. Culture of Specific Cell Types.
24. Culture of Tumor Cells.
25. Organotypic Culture.
26. Scale-Up.
27. Specialized Techniques.
28. Problem Solving.
29. In Conclusion.
Appendix I: Preparation of Reagents.
Appendix II: Sources of Equipment and Materials.
Appendix III: Suppliers and Other Resources.
Appendix IV: Glossary.
Appendix V: General Textbooks and Relevant Journals.
References.
Index.
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