Acknowledgments.

1. Spectroscopic Methods for Matrix Characterization.

2. Enzymes.

3. Quantitation of Enzymes and Their Substrates.

4. Immobilized Enzymes.

5. Antibodies.

6. Quantitative Immunoassays with Labels.

7. Biosensors.

8. Directed Evolution for the Design of Macromolecular Bioassay Reagents.

9. Principles of Electrophoresis.

10. Applications of Zone Electrophoresis.

11. Isoelectric Focusing.

12. Capillary Electrophoresis.

13. Centrifugation Methods.

14. Chromatography of Biomolecules.

15. Mass Spectrometry of Biomolecules.

16. Validation of New Bioanalytical Methods.

Suggested References.

References.

Answers to Problems.

Index.