Culture of Cells for Tissue Engineering
Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure.
Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures.
Features of the text include:
- Easy-to-use format with a two-part organization
- Logically organized—part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues
- Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
- End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book
- A section of elegant color plates to back up the figures in the chapters
Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.
List of Abbreviations.
PART I: CELL CULTURE.
1. Basic Principles of Cell Culture (R. Freshney).
2. Mesenchymal Stem Cells for Tissue Engineering (D. Lennon & A. Caplan).
3. Human Embryonic Stem cell Culture for Tissue Engineering (S. Levenberg, et al.).
4. Cell Sources for Cartilage Tissue Engineering (B. Johnstone, et al.).
5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering (H. Madry).
PART II: TISSUE ENGINEERING.
6. Tissue Engineering: Basic Considerations (G. Vunjak-Novakovic).
7. Tissue Engineering of Articular Cartilage (K. Masuda & R. Sah).
8. Ligament Tissue Engineering (J. Chen, et al.).
9. Cellular Photoencapsulation in Hydrogels (J. Elisseeff, et al.).
10. Tissue Engineering Human Skeletal Muscle for Clinical Applications (J. Shansky, et al.).
11. Engineered Heart Tissue (T. Eschenhagen & W. Zimmermann).
12. Tissue-Engineered Blood Vessels (R. Klinger & L. Niklason).
13. Tissue Engineering of Bone (S. Hofmann, et al.).
14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering (P. Lelkes, et al.).
15. Tissue Engineering of the Liver (G. Underhill, et al.).
R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.
Easy to use format with a two-part organization.
Logically organized, part one discussing cell sourcing, preparation and characterization and the second part looking at specific engineered tissues.
Integrates theoretical background and detailed step-by-step protocols for each system or application.
Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
End-of-chapter features include useful listings of sources for reagents, materials, and supplies
"The editors have brought together an outstanding group of experts to describe cell culture methods and applications for tissue engineering." (Doody's Health Services)