Introduction to Proteomics: Principles and Applications
This book provides a highly authoritative introduction to the promising and fast-advancing field of proteomics, examining the role proteomics plays in the study of biological systems in general and disease in particular. It helps readers understand the structure, function, and interactions of proteins and how this knowledge is used for identifying diseases and developing new drugs.
Nawin Mishra, a world-renowned speaker and leader in proteomics studies who has worked with the late Nobel Laureate E. L. Tatum when proteomics was in its infancy, offers an expert perspective on the entire field, including:
Easily accessible overview of the principles of proteomics
Coverage of real-world, cutting-edge medical applications, including personalized medicine
Clear guidelines on how to operate the complex instrumentation involved in proteomics
Discussion of the future of proteomics
Complete with handy study questions and assuming only a basic understanding of biology, Introduction to Proteomics is an invaluable reference for novices and research scientists alike.
ABOUT THE AUTHOR.
CHAPTER 1 HISTORICAL PERSPECTIVES.
1.1. Introduction to Proteomics.
1.2. Proteome and Proteomics.
1.3. Genetics of Proteins.
1.4. Molecular Biology of Genes and Proteins.
1.5. Protein Chemistry Before Proteomics.
CHAPTER 2 PROTEOMICS—RELATION TO GENOMICS, BIOINFORMATICS.
2.2. Bioinformatics and Computational Biology.
CHAPTER 3 METHODOLOGY FOR SEPARATION AND IDENTIFICATION OF PROTEINS AND THEIR INTERACTIONS.
3.1. Separation of Proteins Via a Multidimensional Approach.
3.2. Determination of the Primary Structure of Proteins.
3.3. Determination of the 3D Structure of a Protein.
3.4. Determination of the Amount of Proteins.
3.5. Structural and Functional Proteomics.
CHAPTER 4 PROTEOMICS OF PROTEIN MODIFICATIONS.
4.1. Phosphorylation and Phosphoproteomics.
4.2. Glycosylation and Glycoproteomics.
4.3. Ubiquitination and Ubiquitinomics.
4.4. Miscellaneous Modifications of Proteins.
CHAPTER 5 PROTEOMICS OF PROTEIN–PROTEIN INTERACTIONS/INTERACTOMES.
5.1. Protein—Protein Interactions (PPI) in Vivo.
5.2. Analysis of Protein Interactions in Vitro.
5.3. Analysis of Protein Interactions in Silico.
5.4. Synthetic Genetic Methods to Determine Protein Interactions.
5.6. Evolution and Conservation of Interactomes.
5.7. Interactomes and the Complexity of Organisms: It is the Number of Interactomes that Matters in Understanding the Complexity of an Organism and not the Number of Genes.
5.8. Interaction of Proteins with Small Molecules.
CHAPTER 6 APPLICATIONS OF PROTEOMICS I: PROTEOMICS, HUMAN DISEASE, AND MEDICINE.
6.2. Medical Proteomics.
6.3. Clinical Proteomics.
6.4. Metaproteomics and Human Health.
6.5. Proteomics in Biotechnology and Industry of Drug Production.
6.6. Metaproteomics of Microbial Fermentation.
6.7. Beef Industry.
6.8. Bioterrorism and Biodefense.
CHAPTER 7 PROTEOMICS—FUTURE DEVELOPMENTS.
7.1. Technical Scope of Proteomics—Beyond Protein Identification.
7.2. Scientific Scope of Proteomics—Control of Epigenesis.
7.3. Medical Scope of Proteomics.
7.4. Proteomics, Energy Production, and Bioremediation.
7.5. Proteomics and Biodefense.
This book provides a highly authoritative introduction to the promising and fast-advancing field of proteomics, examining the role proteomics plays in the study of biological systems in general and disease in particular. It helps readers understand the structure, function, and interactions of proteins and how this knowledge is used for identifying diseases and developing new drugs." (Forbes.com, 1 November 2010)
"For undergraduate and graduate biology and non-biology students and new researchers, Mishra (molecular genetics and genomics, U. of South Carolina) introduces the field of proteomics and its role in the study of biological systems and disease." (Book News, September 2010)