 ELISA and Other Solid Phase Immunoassays: Theoretical and Practical Aspects
ISBN: 978-0-471-90982-8
Paperback
378 pages
January 1991
US $550.00
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BASIC ASPECTS.
An Introduction to ELISA (D. Kemeny & S. Chanter).
Microtitre Plates and Other Solid Phase Supports (D. Kemeny & S. Challacombe).
Quantitative Aspects of Solid Phase Immunoassays (R. Hamilton & N. Atkinson).
AMPLIFICATION.
Amplification by Second Enzymes (A. Johannsson & D. Bates).
The Amplified ELISA (a-ELISA): Immunochemistry and Applications (J. Butler).
MOLECULAR ASPECTS.
The Role of Antibody Affinity in the Performance of Solid Phase Assays (M. Devey & M. Steward).
The Immunochemistry of Sandwich ELISAs: Principles and Applications for the Quantitative Determination of Immunoglobulins (J. Butler).
The Use of ELISA in the Characterization of Protein Antigen Structure and Immune Response (A. Pesce & J. Michael).
The Modified Sandwich ELISA (SELISA) for the Detection of IgE and Other Antibody Isotypes (D. Kemeny).
CELLULAR ELISAs.
The Solid Phase Enzyme-Linked Immunospot Assay (ELISPOT) for Enumerating Antibody-Secreting Cells: Methodology and Applications (C. Czerkinsky, et al.).
ELISA-Plaque Assay for the Detection of Single Antibody-Secreting Cells (J. Sedgwick & P. Holt).
CHEMILUMINESCENCE.
Chemiluminescence Immunoassay (I. Weeks & J. Woodhead).
APPLICATIONS TO MICROBIOLOGY.
The Use of ELISA for Rapid Viral Diagnosis: Viral Antigen Detection in Clinical Specimens (S. Chantler & A. Clayton).
The Use of ELISA for Rapid Viral Diagnosis: Antibody Detection (R. Duncan).
Application of ELISA to Bacteriology (S. Challacombe).
Appendix.
Index.
An Introduction to ELISA (D. Kemeny & S. Chanter).
Microtitre Plates and Other Solid Phase Supports (D. Kemeny & S. Challacombe).
Quantitative Aspects of Solid Phase Immunoassays (R. Hamilton & N. Atkinson).
AMPLIFICATION.
Amplification by Second Enzymes (A. Johannsson & D. Bates).
The Amplified ELISA (a-ELISA): Immunochemistry and Applications (J. Butler).
MOLECULAR ASPECTS.
The Role of Antibody Affinity in the Performance of Solid Phase Assays (M. Devey & M. Steward).
The Immunochemistry of Sandwich ELISAs: Principles and Applications for the Quantitative Determination of Immunoglobulins (J. Butler).
The Use of ELISA in the Characterization of Protein Antigen Structure and Immune Response (A. Pesce & J. Michael).
The Modified Sandwich ELISA (SELISA) for the Detection of IgE and Other Antibody Isotypes (D. Kemeny).
CELLULAR ELISAs.
The Solid Phase Enzyme-Linked Immunospot Assay (ELISPOT) for Enumerating Antibody-Secreting Cells: Methodology and Applications (C. Czerkinsky, et al.).
ELISA-Plaque Assay for the Detection of Single Antibody-Secreting Cells (J. Sedgwick & P. Holt).
CHEMILUMINESCENCE.
Chemiluminescence Immunoassay (I. Weeks & J. Woodhead).
APPLICATIONS TO MICROBIOLOGY.
The Use of ELISA for Rapid Viral Diagnosis: Viral Antigen Detection in Clinical Specimens (S. Chantler & A. Clayton).
The Use of ELISA for Rapid Viral Diagnosis: Antibody Detection (R. Duncan).
Application of ELISA to Bacteriology (S. Challacombe).
Appendix.
Index.
