Preface.

SECTION 1 Agrobacterium-Mediated Transformation.

Chapter 1 Host Factors Involved in Genetic Transformation of Plant Cells by

Agrobacterium (BENOˆIT LACROIX, ADI ZALTSMAN, AND VITALY CITOVSKY).

Chapter 2 Genomics of Agrobacterium–Plant Interaction: An Approach to Refine the Plant Transformation Technology (AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, AND KIRANKUMAR S. MYSORE).

SECTION 2 Other Transformation Technologies.

Chapter 3 Particle Bombardment: An Established Weapon in the Arsenal of Plant Biotechnologists (THEODORE M. KLEIN).

Chapter 4 A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads (NAOKI WADA, SHIN’ICHIRO KAJIYAMA, NARUEMON KHEMKLADNGOEN, AND KIICHI FUKUI).

Chapter 5 Pollen Transformation Technologies (TATIANA RESCH AND ALISHER TOURAEV).

Chapter 6 Intragenic Vectors and Marker-Free Transformation: Tools for a Greener Biotechnology (CAIUS M. ROMMENS, ANTHONY CONNER, HUA YAN, AND ZAC HANLEY).

Chapter 7 Visualizing Transgene Expression (JOHN J. FINER).

SECTION 3 Vectors, Promoters, and Other Tools for Plant Transformation.

Chapter 8 Current State and Perspective of Binary Vectors and Superbinary Vectors (TOSHIYUKI KOMORI AND TOSHIHIKO KOMARI).

Chapter 9 Novel Dual Binary Vectors (pCLEAN) for Plant Transformation (PHILIPPE VAIN).

Chapter 10 pORE Modular Vectors for Plant Transformation (DWAYNE D. HEGEDUS).

Chapter 11 pANIC: A Versatile Set of Gateway-Compatible Vectors for Gene Overexpression and RNAi-Mediated Down-Regulation in Monocots (DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L. ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL STEWART, JR.).

SECTION 4 Transgene Integration, Stability, Methylation, Silencing.

Chapter 12 Understanding and Avoiding Transgene Silencing (MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R. STERNES, AND ROBERT G. BIRCH).

Chapter 13 Site-Specific Recombination for Precise and “Clean” Transgene Integration in Plant Genome (VIBHA SRIVASTAVA AND DAVID W. OW).

SECTION 5 Selection Systems, Marker-Free Transformation.

Chapter 14 Selectable Marker Genes: Types and Interactions (BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE, SOUAD EL OUAKFAOUI, DOUGLAS A. JOHNSON, LORETA GUDYNAITE-SAVITCH, MING HU, AND PHIL MACDONALD).

Chapter 15 Transformation Methods for Obtaining Marker-Free Genetically Modified Plants (JAN G. SCHAART, FRANS A. KRENS, ANNE-MARIE A. WOLTERS, AND RICHARD G.F. VISSER).

Chapter 16 Intellectual Property Aspects of Plant Transformation (JIM M. DUNWELL).

Index.

Color Plate between pages 82 and 83.