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Preservation of Cells: A Practical Manual

ISBN: 978-1-118-98984-5
208 pages
February 2018, Wiley-Blackwell
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Description

Helps those that use cell preservation to develop new protocols or improve existing protocols

 This book provides readers with the tools needed to develop or debug a preservation protocol for cells. The core structure and content of the text grew from a professional short course that has been offered at the Biopreservation Core Resource for the last 10 years. This comprehensive text describes, step by step, the individual elements of a protocol, including the relevant scientific principles for each phase of the protocol. It can be used by anyone who is involved in cell preservation—even by those who are not experts in freezing of cells—because it provides the scientific basis for those that want to understand the basis for the protocol.

Preservation of Cells: A Practical Manual begins by first introducing readers to the subject of preserving cells. It then goes on to cover Pre-freeze Processing and Characterization; Formulation and Introduction of Cryopreservation Solutions; Freezing Protocols; Storage and Shipping of Frozen Cells; Thawing and Post Thaw Processing; Post-thaw Assessment; and Algorithm-driven Protocol Optimization.

  • Clearly explains the reasons behind every step in the development of a preservation protocol and the scientific principles behind them
  • Provides alternative modes of preservation for when conventional methods of cryopreservation are not appropriate for a given cell type or application
  • Enables more organization to achieve improved post thaw recoveries and process consistency

 Preservation of Cells: A Practical Manual is an important book for researchers, laboratory technicians and students in cell biology, stem cell biology, tissue engineering, and regenerative medicine. It is also useful to cell bankers, regenerative medicine, biomarker discovery or precision medicine companies, and cell therapy labs, blood bankers, biobankers, and biotechnology companies.

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Table of Contents

Proposed flow: the organization of the book follows the sequence of protocol development. 

Working table of contents

1. Introduction
a. Applications for preserved cells
b. Elements of preservation protocol

2. Containers
a. Straws
b. Bags
c. Vials
d. Technical requirements
e. Closed system processing

3. Pre-freeze processing
a. Influence of cell culture on freezing response
b. Liquid storage before cryopreservation
c. Selection of subpopulations and other pre-freeze processing processes
d. Evaluating the influence of pre-freeze processing on cell response

4. Formulation of cryopreservation solutions
a. Cryoprotectants
b. Mechanisms of cryoprotection
c. Toxicity of cryoprotective agents
d. Development of introduction and removal protocols for cryopreservation solutions
e. Unique issues associated with formulation of solutions for cellular therapy products

5. Freezing protocol
a. Importance of cooling rate
b. Controlled rate freezing protocols
i. Individual elements of the protocol
ii. Developing the elements of the protocols
c. Passive freezing
i. Development of a passive freezing protocol
ii. Validation of the protocol

6. Storage of frozen cells
a. Scientific basis for selection of a storage temperature
b. Review of scientific literature on storage of cells

7. Thawing
a. Methods of thawing
b. Critical thawing rate
c. Validating your thawing protocol

8. Post thaw assessment
a. Different methods of post thaw assessment
b. Common problem with post thaw assessment
c. Post thaw apoptosis

9. Quality control and continuous assessment

Appendix: sample protocols

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Author Information

Allison Hubel is a Professor of Mechanical Engineering and the Director of BioCoR (Biopreservation Core Resource) at the University of Minnesota, USA.

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