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Human Pluripotent Stem Cells: A Practical Guide

ISBN: 978-1-119-39433-4
176 pages
February 2018, Wiley-Blackwell
Human Pluripotent Stem Cells: A Practical Guide (1119394333) cover image

Description

Comprehensive coverage of the entire induced pluripotent stem cell basic work flow 

Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.

Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.

  • Includes helpful tips and tricks that are normally omitted from regular research papers
  • Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations
  • Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner
  • Written by experts with extensive experience in stem cell technologies

Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.

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Table of Contents

Table of Contents

Aseptic Techniques 

Mammalian Cell Culture Basics

Review of Biochemical Calculations 

Introduction to Stem Cells

PSC Culture

Reprogramming

Differentiation

PSC Characterization

Other Considerations

Appendix I: Reagents, Medias, and Solutions

Appendix II: Common Antibodies

Appendix III: Consumable and Equipment

Appendix IV: Logs and Formas

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Author Information

Uma Lakshmipathy, Director R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Chad C. MacArthur, Staff Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Mahalakshmi Sridharan, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

Rene H. Quintanilla, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA

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