Micropropagation of Orchids, 2 Volume Set, 2nd Edition

Preface to the First Edition.

1. History.

Terminology.

Origins of Orchid Micropropagation.

Culture of Tissues and Organs.

First Micropropagation of Orchids.

The Second Aseptic Culture of an Orchid Explant.

Plant Diseases and Meristems.

The Third Aseptic Culture of an Orchid Explant.

The Fourth Aseptic Culture of an Orchid Explant.

Who Pioneered Micropropagation?.

Root Cultures.

Leaf Cultures.

Stems.

Flower Buds, Flowers, Floral Segments, and Reproductive Organs.

Inflorescences.

A Patent.

Doubtful Claims.

Mutations.

Theft in Vitro.

Darkening of Culture Media.

Anticontaminants.

Cell and Protoplast Culture.

Flowering in Vitro.

The Future.

2. General Outline of Techniques and Procedures.

Media Components.

Stock Solutions.

State of the Medium.

Sterlization.

Surface Decontamination.

Preparation of the Medium.

Culture Vessels.

Culture Conditions.

Placing Plant Material in Culture.

Internal Contaminants.

Enzymes for Protoplast Isolation.

Work Area.

Washing Glassware.

3. Methods for Specific Genera.

Acampe.

Aeridachnis.

Aerides.

Anacamptis.

Angraecum.

Anoectochilus.

Arachnis.

Arachnostylis.

Aranda.

Aranthera.

Arundina.

Ascocenda.

Ascocentrum.

Ascofinetia.

Barlia.

Bletilla.

Brassavola.

Brassia.

Brassocattleya.

Brassolaeliocattleya.

Bulbophyllum.

Burkillara.

Caladenia.

Calanthe.

Catasetum.

Cattleya.

Cleisostoma.

Clowesia.

Cymbidium.

Cypripedium.

Cyrtopodium.

Dactylorchis.

Dactylorhiza.

Darwinara.

Dendrobium.

Disa.

Diuris.

Doriella.

Doritaenopsis.

Doritis.

Encyclia.

Epidendrum.

Epiphronitis.

Eulophia.

Gastrochilus.

Geodorum.

Goodyera.

Grammatophyllum.

Habenaria.

Hetaeria.

Holttumara.

Ipsea.

Kagawara.

Laelia.

Laeliocattleya.

Liparis.

Ludisia (Haemaria).

Luisia.

Lycaste.

Malaxis.

Maxillaria.

Miltonia.

Mokara.

Mormodes.

Neofinetia.

Neostylis.

Neottia.

Nervilia.

Nigritella.

Oberonia.

Odontioda.

Odontoglossum.

Odontonia.

Oncidium.

Ophrys.

Orchis.

Otochilus.

Pachystoma.

Paphiopedilum.

Papilionanthe.

Phaius.

Phalaenopsis.

Pholidota.

Phragmipedium.

Pleione.

Pogonia.

Potinara.

Renades.

Renanetia.

Renantanda.

Renanthera.

Restrepia.

Rhynchostele.

Rhynchostylis.

Saccolabium.

Sarcanthus.

Satyrium.

Schomburgkia.

Serapias.

Sophrolaeliocattleya.

Spathoglottis.

Spiranthes.

Thelymitra.

Thunia.

Trudelia.

Vanda.

Vandofinetia.

Vanilla.

Vascostylis.

Vuylstekeara.

Zygopetalum.

4. Summary.

Culture Conditions.

Media Components.

Applications of Orchid Tissue Culture.

Negative Aspects of Micropropagation.

References.

Appendix 1: General Information on Supplies, Equipment, Terms, and Reagents.

Appendix 2: Sources of Supplies and Equipment.

Appendix 3: Some Sites of Interest on the World Wide Web.

Appendix 4: Light.

Appendix 5: Formulary.

Appendix 6: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions.

Appendix 7: Additional Information.

Appendix 8: Plant Preservative Mixture.

Glossary.

Index.

Title Page of the First Edition