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Micropropagation of Orchids, 2 Volume Set, 2nd Edition

ISBN: 978-1-4051-6088-9
1560 pages
May 2008, Wiley-Blackwell
Micropropagation of Orchids, 2 Volume Set, 2nd Edition (1405160888) cover image
This greatly expanded and updated edition of a classic reference work comprises two volumes offering a compendium of methods for multiplying orchids through micropropagation.
  • A detailed collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro
  • Presents classic techniques that have been in the forefront of orchid propagation since they were first developed in 1949
  • Detailed procedures are appended with tables and complete recipes for a large number of culture media
  • Includes many illustrations, chemical formulas, historical vignettes, and seldom seen illustrations of people, orchids, apparatus and tools

“… an excellent resource like its predecessor, …both informative and captivating, and served as a reminder of why we go to such extremes in our quest to propagate these plants.” American Orchid Society, 2009

“…in the sense of its universal value and importance, this Second Edition will undoubtedly be considered a classic, if only because it will serve as a sole and invaluable resource on the subject.” Plant Science Bulletin, 2009

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Preface.

Preface to the First Edition.

1. History.

Terminology.

Origins of Orchid Micropropagation.

Culture of Tissues and Organs.

First Micropropagation of Orchids.

The Second Aseptic Culture of an Orchid Explant.

Plant Diseases and Meristems.

The Third Aseptic Culture of an Orchid Explant.

The Fourth Aseptic Culture of an Orchid Explant.

Who Pioneered Micropropagation?.

Root Cultures.

Leaf Cultures.

Stems.

Flower Buds, Flowers, Floral Segments, and Reproductive Organs.

Inflorescences.

A Patent.

Doubtful Claims.

Mutations.

Theft in Vitro.

Darkening of Culture Media.

Anticontaminants.

Cell and Protoplast Culture.

Flowering in Vitro.

The Future.

2. General Outline of Techniques and Procedures.

Media Components.

Stock Solutions.

State of the Medium.

Sterlization.

Surface Decontamination.

Preparation of the Medium.

Culture Vessels.

Culture Conditions.

Placing Plant Material in Culture.

Internal Contaminants.

Enzymes for Protoplast Isolation.

Work Area.

Washing Glassware.

3. Methods for Specific Genera.

Acampe.

Aeridachnis.

Aerides.

Anacamptis.

Angraecum.

Anoectochilus.

Arachnis.

Arachnostylis.

Aranda.

Aranthera.

Arundina.

Ascocenda.

Ascocentrum.

Ascofinetia.

Barlia.

Bletilla.

Brassavola.

Brassia.

Brassocattleya.

Brassolaeliocattleya.

Bulbophyllum.

Burkillara.

Caladenia.

Calanthe.

Catasetum.

Cattleya.

Cleisostoma.

Clowesia.

Cymbidium.

Cypripedium.

Cyrtopodium.

Dactylorchis.

Dactylorhiza.

Darwinara.

Dendrobium.

Disa.

Diuris.

Doriella.

Doritaenopsis.

Doritis.

Encyclia.

Epidendrum.

Epiphronitis.

Eulophia.

Gastrochilus.

Geodorum.

Goodyera.

Grammatophyllum.

Habenaria.

Hetaeria.

Holttumara.

Ipsea.

Kagawara.

Laelia.

Laeliocattleya.

Liparis.

Ludisia (Haemaria).

Luisia.

Lycaste.

Malaxis.

Maxillaria.

Miltonia.

Mokara.

Mormodes.

Neofinetia.

Neostylis.

Neottia.

Nervilia.

Nigritella.

Oberonia.

Odontioda.

Odontoglossum.

Odontonia.

Oncidium.

Ophrys.

Orchis.

Otochilus.

Pachystoma.

Paphiopedilum.

Papilionanthe.

Phaius.

Phalaenopsis.

Pholidota.

Phragmipedium.

Pleione.

Pogonia.

Potinara.

Renades.

Renanetia.

Renantanda.

Renanthera.

Restrepia.

Rhynchostele.

Rhynchostylis.

Saccolabium.

Sarcanthus.

Satyrium.

Schomburgkia.

Serapias.

Sophrolaeliocattleya.

Spathoglottis.

Spiranthes.

Thelymitra.

Thunia.

Trudelia.

Vanda.

Vandofinetia.

Vanilla.

Vascostylis.

Vuylstekeara.

Zygopetalum.

4. Summary.

Culture Conditions.

Media Components.

Applications of Orchid Tissue Culture.

Negative Aspects of Micropropagation.

References.

Appendix 1: General Information on Supplies, Equipment, Terms, and Reagents.

Appendix 2: Sources of Supplies and Equipment.

Appendix 3: Some Sites of Interest on the World Wide Web.

Appendix 4: Light.

Appendix 5: Formulary.

Appendix 6: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions.

Appendix 7: Additional Information.

Appendix 8: Plant Preservative Mixture.

Glossary.

Index.

Title Page of the First Edition

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Dr Joseph Arditti majored in floriculture as an undergraduate and received his doctorate from the University of Southern California in 1965. After serving as a lecturer for one year at the University of Southern California, he accepted a faculty position at the University of California, Irvine in 1966 where he taught General Botany, Horticulture, and Plant Physiology while engaging in research on various aspects of orchid biology in the US, Indonesia, Singapore, and Malaysia. Dr Arditti retired in 2001 and is now Professor Emeritus. He continues to write and travel worldwide to lecture at scientific meetings and to orchid groups. He is acknowledged to be one of the world's leading experts on orchid biology and propagation.
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  • A detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro
  • Presents classic techniques that have been at the forefront of orchid propagation since they were first developed in 1949, as well as the latest cutting-edge methodology
  • Detailed procedures are appended with tables and complete recipes for a large number of culture media
  • Includes many illustrations, chemical formulas, and historical vignettes
See More
"It contains a great amount of information including the most up to date scientific results. Micropropagation of Orchids, 2nd edition, is a valuable book of reference for people working in the area of orchid cultivation and for all who are enthusiastic with orchids." (Thaiszia Journal of Botany, 2010)

"This title comes as two separate hardcover volumes, attractively housed together within a 5-sided cardboard casing that matches the volume covers. This second edition expands and updates material registered in the first edition, which was published in 1993 by Wiley and Sons and covered orchid micropropagation methods published between 1974 and 1990 within 682 pages. The second edition boasts another 878 pages, accounting for the large increase in methods published across the years 1990 and 2000. Reviewers' comments indicate that this publication continues to be a primary resource for this discipline." (Journal of the Botanical Research Institute of Texas, 2010)

"My minor worries do not stop me wholeheartedly recommending people actively involved in orchid micropropagation and institutional librarians to purchase the second edition of what will, I am sure, be the standard work on the subject for years to come." (Annals of Botany, April 2010)

"The book lives up to its billing as a useful resource strictly dedicated to micropropagation ... .Secure a copy for your personal library before it is out of print once and for all." (American Orchid Society, 2009)

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