Preface.

Part I: Fluorescence in High-Throughput Screening.

1. Single Beads, Single Molecule, Single Cell Fluorescence.

2. Substrate Arrays for Fluorescence-Based Enzyme Fingerprinting and High-Throughput Screening.

Part II: Fluorescence Spectroscopy.

3. Probing the Interior of Living Cells with Fluorescence Correlation Spectroscopy.

4. Fluorescence Measurements on Functionalized Polymer Surfaces-- Problems and Troubleshooting.

5. Standardization of Fluorescence Measurements--Criteria for the Choice of Suitable Standards and Approaches to Fit-for-Purpose Calibration Tools.

6. Investigating Transcriptional Regulation by Fluorescence Spectroscopy, from Traditional Methods to State-of-the-Art Single Molecule Approaches.

7. Sub-picosecond Transient Signal Spectroscopy of Prodan in DMF Solution.

8. Frequency Domain Fluorometry with Pulsed LEDs.

9. Towards Single Metal Ion Sensing by Forster Resonance Energy Transfer.

10. Fluorescence Interferometry.

11. Acoustically Levitated Droplets.

12. Structural Effects of Biologically Relevant Rhodamines on Spectroscopy of Fluorescence Fluctuations.

Part III: Fluorescence Imaging.

13. Dextrin Microencapsulated Porphyrin.

14. Lanthanide Bimetallic Helicates for in vitro Imaging and Sensing.

15. Time-Gated Luminescence Microscopy.

16. Time-resolved Microspectrofluorometry and Fluorescence Imaging Techniques.

Part IV: Fluorescence Probes and Labels.

17. Fluorescent DNA Base Modifications and Substitutes.

18. Fluorescent Probes for Chemical Transformations on the Single-Molecule Level.

19. Luminescent Lanthanide Complexes as Probes for the Determination of Enzyme Activities.

20. Which Are You Watching, an Individual ROS or Total Oxidative Stress?.

21. Probing of Co-solvents in Polymer Latex Materials using Solvatochromic Fluorescence.

22. Red/Near-Infrared Boron-dipyrromethene (BODIPY) Dyes as Strongly Emitting Fluorophores.

23. Sensitive Terbium Probes for Luminescent Determination of both Alkaline Phosphatase and Codeine Phosphate.

24. Fluorescent Probes and Labels for Biomedical Applications.

Part V: Fluorescence Micro- and Nanoparticles.

25. Photon Upconversion in Homogeneous Fluorescence-Based Bioanalytical Assays.

26. Processing and Characterization of Au Nanoparticles for Use in Plasmon Probe Spectroscopy and Microscopy of Biosystems.

27. Multi-functionalized Biocompatible Microspheres for Sensing.

28. Fluorescent Silica Nanoparticles.

29. Thermally Activated Delayed Fluorescence in Fullerenes.

30. Stability and Fluorescence Quantum Yield of CdSe/ZnS Quantum Dots--Influence of the Thickness of the ZnS-shell.

31. Size Distributions of CdS Nanoparticles Obtained from Templating Methods.

32. Excitation Energy Transfer and Trapping in Dye-loaded Solid Particles.

33. Characterization of Water-soluble Luminescent Quantum Dots by Fluorescence Correlation Spectroscopy.

34. Luminescent Amino-functionalized or Erbium-doped Silica Spheres for Biological Applications.

35. Preparation and Characterization of Nanocrystalline ZrO2:Yb3+,Er3+ Up-conversion Phosphors.

36. Nano- and Microparticles of Organic Fluorescent Dyes.

Part VI: Biophysical Fluorescence and Bioanalytical Fluorescence.

37. Flow Cytometric FRET Analysis of erbB Receptor Interaction on a Cell-by-Cell Basis.

38. Rolling Circle Amplification A New Detection Method for the Sensitive Measurement of Interactions on Biochips.

39. Fluorescent Studies on Cooperative Binding of Cationic Pheophorbide-a Derivative to Polyphosphate.

40. The Effect of pH on Aqueous Phenylalanine Studied Using a 265 nm Pulsed Light-emitting Diode.

41. Ordered Self-assembly of Protonated Porphyrin Induced by the Aqueous Environment of Biomimetic Systems.

42. Human Serum Albumin-Flavonoids Interactions Monitored by Means of Tryptophan Kinetics.

43. Two-photon Excitation Fluorescence Bioassays.