Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution
From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.
Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.
As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.
In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.
Generation of Mutant Libraries Using Random Mutagenesis
DNA Recombination Using StEP
FACS Screening of Combinatorial Peptide and Protein Libraries
Selection of Phage-displayed Enzymes
Selection of Aptamers
Methods for Selecting Catalytic Nucleic Acids
High-Throughput Screening of Enantioselective Industrial Biocatalysts
Computer-assisted Design of Doped Libraries
Directed in silico Mutagenesis
RNA Folding in silico
Patenting in Evolutionary Biotechnology
Programs on CD: How to identify positions suited for mutagenesis;
Folding aspects; databases; structure-based design; and more
Glossary and Index
Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Göttingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Münster and Göttingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Göttingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.
"... contains valuable information." Molecular Biotechnology
"... is truly a highly practical handbook, a "must have" for any laboratory practicing directed evolution of proteins, and a methodological "cookbook" of high quality." Angewandte Chemie I.E.
"...this volume can be recommended to novices desirous to get started with evolutionary methods as well as experienced scientists of the field." Carbohydrate Polymers
"... a useful snapshot of the state of the art of directed evolution methods for academia and industrial working groups." The Quarterly Review of Biology
"... a terrific collection of useful tricks and protocols that can be wholeheartedly recommended." ASM News
Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution (US $260.00)
-and- Student Companion to Accompany Fundamentals of Biochemistry, 4th Edition (US $89.95)
Total List Price: US $349.95
Discounted Price: US $262.46 (Save: US $87.49)