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More Practical Problem Solving in HPLC

ISBN: 978-3-527-31113-2
309 pages
January 2005
More Practical Problem Solving in HPLC (3527311130) cover image
A unique approach to solving HPLC problems.
Everyone who bought "Problem Solving in HPLC" by Stavros Kromidas will equally benefit from nearly 100 new practical examples for optimization, trouble-shooting, and instrument performance given in this sequel.
The author provides
- guidance for selecting and evaluating methods, intstruments and columns,
- practical help with everyday trouble-shooting,
- advice for optimizing separations, always explaining the reason why.
In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in addition the book contains manufacturers' addresses, references, data tables and checklists.
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In Lieu of an Introduction
Crosswords
A Puzzle
An HPLC Tale
HPLC TIPS
Stationary Phases, Columns (Tips 1-11)
Buffers, pH Value (Tips 12-21)
Optimization, Peak Homogeneity (Tips 22-37)
Troubleshooting (Tips 38-55)
Have A Break...
Complete the Sentences
Matching Pairs/Statements
Did Peaky Remember His Lesson Correctly?
General HPLC Tips (Tips 56-73)
Literature HPLC Tips
LC-MS COUPLING, NANO AND MICRO LC
LC-MS Coupling (Tips 74-81)
Micro And Nano LC (Tips 82-91)
Quantification
APPENDIX
Solutions to Quantification Problems
From Theory to Practice -
Empirical Formulae, Rules of Thumb and Simple Relationships in Everyday HPLC
Information Resources for Analysis/HPLC
Analytical Chemistry Today
Trends in HPLC
Thoughts about a Dead Horse
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Stavros Kromidas worked as Sales Manager for Waters Chromatography following his PhD thesis completed in 1983. From 1989 to 2001 he was Managing Director of Novia GmbH, a consultant company for analytical chemistry and is an independent consultant since then. He has worked in the area of HPLC since 1978 and has given lectures and training courses since 1984. He is author and co-author of several articles and numerous successful books.
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  • The fast way for solving HPLC problems! A unique approach to solving HPLC problems.
  • Nearly 100 new practical examples for optimisation, trouble-shooting, and instrument performance in this book.
  • Offers guidance for selecting and evaluating methods, intstruments and columns
  • Gives practical help with everyday trouble-shooting,
  • Gives advice for optimizing separations, always explaining the reason why.
  • In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in addition the book contains manufacturers' addresses, references, data tables and checklists. This unique approach cannot be found in any other HPLC book.
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"The book is very clearly written, clearly arranged, clearly laid out and concise. ... the book holds valuable and applicable information, which make it a useful reference book for analytical questions/problems and their answers/solutions."
Physical Sciences Educational Reviews

"This book is very readable and written in a light mood...suitable as a reference for scientists utilizing these associated methods...Highly recommended."
CHOICE

"Thanks go to the author for sharing a quantity and depth of knowledge that can be obtained only from decades immersed in the field."
Journal of Natural Products
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