 Artificial Enzymes
ISBN: 978-3-527-31165-1
Hardcover
193 pages
September 2005
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List of Authors.
1 Artificial Enzymes (Ronald Breslow).
1.1 Mimics of Enzymes that use Thiamine Pyrophosphate as a Coenzyme.
1.2 Mimics of Enzymes that use Pyridoxamine and Pyridoxal Phosphates as Coenzymes.
1.3 Artificial Hydrolytic Enzymes.
1.4 Cytochrome P-450 Mimics.
1.5 Future Prospects.
2 Vitamin B6 Enzyme Models (Lei Liu and Ronald Breslow).
2.1 Introduction.
2.2 Transamination.
2.3 Racemization.
2.4 Decarboxylation.
2.6 Aldolase-type Reactions.
3 Evolution of Synthetic Polymers with Enzyme-like Catalytic Activities (Irving M. Klotz and Junghun Suh).
3.1 Introduction: Conceptual Background.
3.2 Homogeneous Polymer Biocatalysts.
3.3 Heterogeneous Polymer Biocatalysts.
3.4 Prospectives.
4 Mimicking Enzymes with Antibodies (Donald Hilvert).
4.1 Introduction.
4.2 Basic Strategy.
4.3 Evolution of Binding Affinity and Catalytic Efficiency.
4.4 Importance of a Good Fit.
4.5 General Acid–General Base Catalysis.
4.6 Covalent Catalysis.
4.7 Practical Applications.
4.8 Future Directions.
4.9 Outlook.
5 Protein-based Artificial Enzymes (Ben Duckworth and Mark D. Distefano).
5.1 Introduction.
5.2 Artificial Nucleases Based on DNA and RNA Binding Proteins.
5.3 Catalysts Based on Hollow Lipid-binding Proteins.
5.4 Myoglobin as a Starting Point for Oxidase Design.
5.5 Antibodies as Scaffolds for Catalyst Design.
5.6 Conclusions.
6 Artificial Hydrolytic Metalloenzymes (Jik Chin and Hae-Jo Kim).
6.1 Introduction.
6.2 Reactivity of Substrates.
6.3 Lewis Acid Activation.
6.4 Nucleophile Activation.
6.5 Leaving-group Activation.
6.6 Combining Lewis Acid Activation and Nucleophile Activation.
6.7 Double Lewis Acid Activation.
6.8 Phosphatase Models.
6.9 Phosphodiesterase Models.
6.10 Polymerases and DNases.
6.11 Conclusion.
7 Artificial Restriction Enzymes As Tools For Future Molecular Biology and Biotechnology (Yoji Yamamoto and Makoto Komiyama).
7.1 Introduction.
7.2 Significance of Artificial Restriction Enzymes.
7.3 Non-enzymatic Catalysts for DNA Hydrolysis.
7.4 Molecular Design of Artificial Restriction Enzymes (Covalent vs. Non-Covalent Strategy).
7.5 Site-selective Scission of Single-stranded DNA.
7.6 Site-selective Scission of Double-stranded DNA by Combining Ce(IV)/EDTA Complex with Pseudo-complementary PNA.
7.7 Conclusion.
Index.
