1 Developmental processes.

2 Cells and Tissue.

2.1 The Cell.

2.2 Tissue Types.

2.3 Relevance of the ECM.

2.4 Emergence of Tissue.

2.5 Regeneration.

3 Classical Culture Methods.

3.1 History.

3.2 First Cultures.

3.3 Tissue Culture.

3.4 Organ Culture.

4 Tissue Engineering.

4.1 Cell Therapies.

4.2 Tissue Constructs.

4.3 Organ Modules.

4.4 Cosmetic Measures.

5 Concepts of Tissue Creation.

5.1 Sources.

5.2 Stem Cells.

5.3 Cells from Tissues.

5.4 Matrices.

5.5 Culture Methods for Tissue Engineering.

5.6 Perfusion Culture.

6 Maturation of Tissue Constructs.

6.1 Primary and Secondary Contacts.

6.2 Building Structures.

6.3 Terminal Differentiation.

6.4 Impact of the Culture Environment on the Development of Tissue.

6.5 Step by Step.

6.6 Tissue Functions after Implantation.

6.7 The Three Steps of Tissue Development.

7 Development of the Perfusion System Tissue Factory.

7.1 Requirements of the Culture System.

7.2 Artificial Interstitium.

7.3 Smart Matrices.

7.4 Optimal Housing for the Perfusion System.

7.5 Supply of the Maturing Tissue with Medium.

7.6 Synopsis.

8 Ensuring Tissue Quality.

8.1 Norms and Cell Biology.

8.2 Evaluating Complexity.

8.3 Expression Behavior.

8.4 Suitability of a Scaffold.

8.5 Hidden Heterogeneity.

8.6 Investigating Cellular Ultrastructures.

8.7 Functional Transfer.

8.8 Quality Assurance.

8.9 Implant–Host Interaction.

9 Perspectives.

10 Ethical Aspects.

Glossary.

Companies.

Literature.

Subject Index.