Applied Thin-Layer Chromatography: Best Practice and Avoidance of Mistakes, 2nd Edition
Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures.
How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.
What is TLC?
When is TLC used?
Where is TLC used
How is the result of a TLC represented?
What kinds of reference substances are used in TLC?
The literature on TLC
Why use precoated layers?
How are precoated layers produced?
What kinds of precoated layers are there?
What are precoated layers used for?
Criteria for choosing stationary phases in TLC
Effect of the stationary phase when the solvents are identical
Recommendations for the procurement and storage of precoated layers
Problems in the nomenclature and classification of precoated layers
BEFORE TLC DEVELOPMENT
Handling of the precoated layers
Application of the sample
Positioning of the sample
Drying before development
SOLVENT, DEVELOPING CHAMBERS AND DEVELOPMENT
TLC developing chambers
Development of thin-layer chromatograms
Drying after development
EVALUATION WITHOUT DERIVATIZATION
Visual direct evaluation
Optical direct evaluation using special equipment
Coupling methods for substance identification
Documentation without or before derivatization
Irradiation with high energy light
Reaction with reagents
Special types of derivatization
Treatment of derivatized chromatograms
EVALUATION AFTER DERIVATIZATION
Evaluation by TLC scanner
Evaluation by video system
Evaluation by flat-bed scanner
Description of a thin-layer chromatogram
Documentation by reproduction, tracing or photocopying
TLC scanner documentation
Flat-bed scanner documentation
GMP/GLP-CONFORMING TLC WORK
Validation of TLC methods
Use of qualified
GMP/GLP-conforming rawdata sheets
Examples of GMP/GLP-conforming testing procedures
Definite loading of a substance
TLC of sensitive substances
SPECIAL METHODS IN TLC
Automated Multiple Development
Overpressured Layer Chromatography
High Pressure Planar Liquid Chromatography
Combination of TLC with Flame Ionization Detector or Flame Thermoionic Ionization Detector
Rotation Planar Chromatography
Chromatography and art
"This impressive book thoroughly discusses a subject not usually covered thoroughly in the analytical chemistry literature." (CHOICE, June 2007)
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Applied Thin-Layer Chromatography: Best Practice and Avoidance of Mistakes, 2nd Edition (US $237.00)
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