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Biophysical Analysis of Membrane Proteins: Investigating Structure and Function

ISBN: 978-3-527-31677-9
368 pages
December 2007, Wiley-Blackwell
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Meeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights.
Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in whole cells.
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Table of Contents

Membrane Protein Structure Determination by Electron Cryo-microscopy
NMR Liquid or Solid State
Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent
SPR Biosensors for Characterizing Membrane-associated Proteins
Atomic Force Microscopy (AFM): High Resolution Imaging of Structure and Assembly of Membrane Proteins
Molecular Dynamics Simulations
Understanding Structure and Function of Membrane Proteins Using Free Energy Calculations
Neutron Scattering to Study Membrane Structure and Dynamics
Circular Dichroism: Folding and Conformational Changes of Membrane Proteins
Membrane Protein Structure and Conformational Change Probed Using Fourier Transform Infrared Spectroscopy
Raman Spectroscopy on Light Harvesting Complex
Energy Transfer Technologies to Monitor the Dynamics and Signaling Properties of G-protein Coupled Receptors in Living Cells
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Author Information

Professor Eva Pebay-Peyroula carried out her PhD in molecular physics in the SNCI-CNRS Grenoble in 1986, where she continued to work during her post-doc. In 1991 she was made professor at the Université Joseph Fourier, and in 2001 set up the Membrane Protein Laboratory in the Institute for Structural Biology, Grenoble, where she also serves as director of the institute. In 2004 she was elected a member of the French Academy of Science, and she has won the Université Joseph Fourier gold medal award in 2004 and the silver medal of the CNRS in 2005.
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"...exemplified by the respective author’s own studies…" (Wiley-VCH - ChemBioChem)
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