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Enzyme Kinetics: Principles and Methods, Second, Revised and Updated Edition

ISBN: 978-3-527-31957-2
320 pages
May 2008, Wiley-Blackwell
Enzyme Kinetics: Principles and Methods, Second, Revised and Updated Edition (3527319573) cover image
This new, expanded and updated edition of the user-friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.
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Symbols and Abbreviations
Introduction
Multiple Equilibria
- Diffusion
- Interaction of ligands with macromolecules
o Macromolecules with identical, non-identical, independent and interacting binding sites
Enzyme Kinetics
- reaction order
- kinetics,
o analysis of kinetic data, enzyme inhibition, multi-substrate reactions,
o analysis of complex enzyme mechanisms, allosteric enzymes,
- PH, temperature dependence of enzymes
- Isotope exchange
- Statistical methods in enzyme kinetics
Methods
- methods for the investigation of multiple equilibria, electrochemical methods, spetroscopy, calorimetry, measurements of fast reactions
Index
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Prof. Dr. Hans Bisswanger is currently at the Interfaculty Institute of Biochemistry at the University of Tübingen. He has developed and leads an intensive course on enzyme kinetics, enzyme technology and ligand binding, while his main focus is on structural and regulatory mechanisms of multi-enzyme complexes and temperature stable enzyme complexes. He is also working on the gentle immobilization of enzyme activities on synthetic surfaces, a method which has been favorably applied for the restoration of damaged documents.
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“Overall, this is an excellent and thorough treatment of a difficult subject. As well as appealing to biochemists, there are also elements of interest to those studying pharmacology and the pharmacokinetic distribution of drugs. The mathematical nature and advanced coverage means that it is most suitable for researchers who already have a strong grounding in the subject. I will certainly be consulting it during my research.”  (Biochemist, 29 May 2013)

“This new, expanded and updated edition of the user–friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.”  (Biology Community, 29 November 2012)

Enzyme kinetics is not a new area in biochemistry. Thirty years ago, enzyme kinetics was one of the most important tools for deconstructing enzymatic mechanisms. With advances in enzyme structure determination and molecular genetics, enzyme kinetics is no longer as prominent. However, enzyme kinetics is still useful to gain insight into enzymes that are too large for NMR studies and that cannot be crystallized. Many enzymes that fit into this category are membrane bound, the kinetics of which are much more complicated. In this new edition (1st ed., 2002), Bisswanger (Univ. of Tübingen, Germany) does a nice job of extending solution enzyme kinetics to membrane-bound enzymes. Setting it apart from other works on the subject, Enzyme Kinetics does not simply deal with substrate binding as a part of the reaction kinetics, but instead devotes about one-third of the text to equilibrium binding between macromolecules and ligands in which no reaction catalysis follows the binding. This binding is then directly connected with enzyme catalyzed reaction kinetics. Enzyme Kinetics was written to serve as a graduate-level course resource and would serve this population well. The inclusion of student problems would be an improvement. Summing Up: Recommended. Graduate students, researchers, and faculty. -- L. J. Liotta, Stonehill College (Choice, February 2009)
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Enzyme Kinetics: Principles and Methods, Second, Revised and Updated Edition (US $247.95)

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