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Infrared and Raman Spectroscopic Imaging

Reiner Salzer (Editor), Heinz W. Siesler (Editor)
ISBN: 978-3-527-31993-0
530 pages
May 2009
Infrared and Raman Spectroscopic Imaging (352731993X) cover image
A comparison of the strengths and weaknesses of near-infrared, infrared and Raman imaging, focusing on current as well as conceivable applications for chemical analysis in delicate natural and synthetic samples. This handbook and ready reference covers instrumentation for vibrational spectroscopic imaging, chemometric evaluation of spectroscopic images, and vibrational spectroscopic imaging in biology and medicine, as well as the chemical, pharmaceutical and food industries.
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PART I: Basic Methodology

INFRARED AND RAMAN INSTRUMENTATION FOR MAPPING AND IMAGING
Introduction to Mapping and Imaging
Mid-Infrared Microspectroscopy and Mapping
Raman Microspectroscopy and Mapping
Near-Infrared Hyperspectral Imaging
Raman Hyperspectral Imaging
Mid-Infrared Hyperspectral Imaging
Mapping with Pulsed Terahertz Radiation
CHEMOMETRIC TOOLS FOR IMAGE ANALYSIS
Introduction
Hyperspectral Images: The Measurement
Image Preprocessing
Exploratory Image Analysis
Quantitative Image Information : Multivariat Image Regression (MIR)
Image Segmentation
Image Resolution

PART II: Biomedical Applications

VIBRATIONAL SPECTROSCOPIC IMAGING OF SOFT TISSUE
Introduction
Preparation of Soft Tissue for Vibrational Spectroscopic Imaging
Applications to Soft Tissues
VIBRATIONAL SPECTROSCOPIC IMAGING OF HARD TISSUE
Infrared and Raman Spectroscopy of Bone
Infrared and Raman Imaging of Bone
Infrared and Raman Spectroscopy of Cartilage
Infrared and Raman Imaging of Cartilage
INFRARED AND RAMAN SPECTROSCOPY AND SPECTRAL IMAGING OF INDIVIDUAL CELLS
Introduction
Methods
Results and Discussion
3-D IMAGING OF BIOMEDICAL SAMPLES
Introduction
Methodologies
Resultant 3-D Images

PART III: Agricultural Applications

FT-IR MICROSPECTROSCOPIC IMAGING OF PLANT MATERIAL
Introduction, Background and Perspective
Application of IMS to Kernels and Seeds of Plants
Leaves
Application of IMS to Stems and Cellulose Polymers
Algae
NEAR-INFRARED HYPERSPECTRAL IMAGING IN FOOD AND AGRICULTURAL SCIENCE
Introduction
At the Laboratory Scale
At the Industrial Scale

PART IV: Polymers and Pharmaceuticals

FT-IR AND NIR SPECTROSCOPIC IMAGING: PRINCIPLES, PRACTICAL ASPECTS AND APPLICATIONS IN MATERIAL AND PHARMACEUTICAL SCIENCES
Introduction
Instrumentation for FT-IR and NIR Imaging
Applications of FT-IR Imaging for Polymer Research and Quality Control
NIR Imaging Spectroscopy for Quality Control of Pharmaceutical Drug Formulations
ATR-FT-IR IMAGING FOR PHARMACEUTICAL AND POLYMERIC MATERIALS: FROM MICRO TO MACRO APPROACHES
Introduction
The Versatility of ATR-FT-IR Imaging
Applications of ATR-FT-IR Imaging in the Materials Sciences
Applications of ATR-FT-IR Imaging in the Pharmaceutical Sciences
ATR-FT-IR Imaging for Forensic Applications
RAMAN SPECTRAL IMAGING ON PHARMACEUTICAL PRODUCTS
Overview
Hyperspectral Imaging
Empirical Approach to Successful Raman Hyperspectral Imaging
Raman in Multimodal Hyperspectral Imaging

PART V: Developments in Methodology

DEEP, NONINVASIVE RAMAN SPECTROSCOPY OF DIFFUSELY SCATTERING MEDIA
Introduction
Techniques for Deep, Noninvasive Raman Spectroscopy
Examples of Application Areas
Outlook on Raman Tomography
PLANAR ARRAY INFRARED SPECTROSCOPY AND 1-D IMAGING
Introduction
Concept and Instrumentation
PA-IR Spectroscopy
Applications
BIOMEDICAL APPLICATIONS OF INFRARED MICROSPECTROSCOPY USING SYNCHROTRON RADIATION
Introduction
Spatial Resolution Considerations
Advantages of a Synchrotron IR Source
Instrumentation
Biological Sample Preparation and Modes of Data Collection
Biological and Medical Applications on Synchrotron IR Microspectroscopy
Future Directions for Synchrotron IR Microspectroscopy
SPECTROSCOPIC IMAGING WITH NANOMETER RESOLUTION USING NEAR-FIELD METHODS
Introduction
Methods
Applications
Current Challenges

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Reiner Salzer retired as Professor of Analytical Chemistry at the Technische Universität Dresden, Germany. He obtained his academic degrees from the University of Leipzig, before taking up his appointment at the TU Dresden. He has authored over 250 books and scientific publications and was elected onto various national and international boards. Professor Salzer is a member of the Norwegian Academy of Science and, a recipient of the Emich Plaque of the Austrian Society of Analytical Chemistry. He is a long-time advisory board member for analytical and spectroscopic journals. Professor Salzer served as President of the Division Analytical Chemistry of the German Chemical Society and is National Delegate to the Division Analytical Chemistry of EuCheMS, where he is Head of the Study Group Education. He was elected as a member of the ECTNA Label Committee for the Chemistry Eurobachelor and Chemistry Euromaster.

Heinz W. Siesler has been a professor of Physical Chemistry at the University of Duisburg-Essen, Germany, since 1987. After receiving his PhD in chemistry in 1970 from the University of Vienna, Austria, he worked as a post-doc at the University of Cologne, Germany, and at the Witwatersrand University, Johannesburg, South Africa. Prior to his present position he gained industrial experience as section head in molecular spectroscopy and thermal analysis in the Corporate R&D Department of Bayer AG, Germany. Between 1992 and 2008 he held guest professorships in France, Japan, and Austria. Professor Siesler is a recipient of the EAS Award, the Tomas Hirschfeld Award and the Buechi Award in near-infrared spectroscopy. His main research focuses on the application of vibrational spectroscopy to chemical and polymer research, analysis and quality control, and he has written over 190 publications in this field.
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"The book provides a full overview of the potential of vibrational spectroscopy in biology, food, and pharmaceutical fields. The instrumentation, data processing, applications, and recent developments are presented. The book is clearly written in a comprehensive manner. It represents a valuable resource for everyone working in IR and/or Raman microimaging." (Anal Bioanal Chem, 2010)

"An especially positive aspect of the book is the strong emphasis on practical relevance in the chapters. ... Recommended both to method developers and to applications practitioners in academic and industrial laboratories." (Angewandte Chemie International Edition, February 2010)

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