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HPLC Made to Measure: A Practical Handbook for Optimization

ISBN: 978-3-527-61199-7
786 pages
June 2008
HPLC Made to Measure: A Practical Handbook for Optimization (3527611991) cover image


The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness.
This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.
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Table of Contents

Principles of HPLC Optimization Illustrated by RP-Chromatography
Fast Gradient Separations
pH and Selectivity in Reversed-Phase Chromatography
Selecting the Correct pH-Value for HPLC
Optimization of Data Evaluation in Chromatography
Calibration Characteristics and Uncertainty indicating Starting-Points for Method Optimization
Comparison and Choice of Columns for RP-HPLC
Column Selectivity in Reversed-Phase Chromatography
The Use of Principal Component Analysis for the Characterisation of Reversed-Phase Liquid Chromatographic Stationary Phases
Chemometrics -
A Powerful Tool for Handling a Large Number of Data
Linear Free Energy Relations -
Tools for Column Characterization and Method Optimization in HPLC?
Column Selectivity in Reversed-Phase Liquid Chromatography
Understanding Selectivity by Use of Suspended-State High Resolution Magic Angle Spinning NMR Spectroscopy
Optimization in Normal-Phase HPLC
Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection
Gel Filtration -
Size Exclusion Chromatography of biopolymers
Optimization in Affinity Chromatography
Optimization of Chiral Separations in HPLC
?LC/nano LC -
Optimization and Troubleshooting
Microchip-Based Liquid Chromatography -
Techniques and Possibilities
Ultra-Performance Liquid Chromatography
Immunochromatographic Techniques
Enhanced Characterization and Comprehensive Analyses by 2-dimensional Chromatography
Hints and Recommendations for Optimization and Troubleshooting
Computer-Facilitated HPLC Method Development Using DryLab Software
ChromSword Software for Automated and Computer-Assisted Development of HPLC Methods
Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC
NanoLC-MS/MS in Proteomics
Ways for the Verification of the Robustness in RP-HPLC
Separation of Complex Sample Mixtures
Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC-UV/MS/CLND
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Author Information

Following his PhD thesis in 1983, Stavros Kromidas worked as a sales manager for Waters Chromatography . From 1989 to 2001 he was Managing Director of Novia GmbH, a consultancy company for analytical chemistry, and he has been an independent consultant since then. He has been working in the field of HPLC since 1978 and has given lectures and training courses since 1984. He is the author and co-author of numerous articles and several successful books.
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"A valuable text by any estimation, I would recommend it highly for hands-on users or managers directing a group of chromatographers toward more efficient and effective HPLC separations." (Journal of the American Chemical Society, February 21, 2007)
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