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Microcharacterization of Proteins, 2nd Edition

ISBN: 978-3-527-61397-7
346 pages
September 2008, Wiley-Blackwell
Microcharacterization of Proteins, 2nd Edition (3527613978) cover image

Description

Proteomics - the analysis of the whole set of proteins and their functions in a cell - is based on the revolutionary developments which have been achieved in protein analysis during the last years.
The number of finished genome projects is growing and in parallel there is a dramatically increasing need to identify the products of revealed genes. Acting on a micro level modern protein chemistry increases our understanding of biological events by elucidating the relevant structure-function relationships.
The second edition of the successful title Microcharacterization of Proteins presents a current overview of modern protein analysis: From sample preparation to sequence analysis, mass spectrometry and bioinformatics it informs about the tools needed in protein research. This makes the book indispensable for everyone involved in proteomics!
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Table of Contents

SECTION I: OVERVIEW
Microcharacterization of Proteins
SECTION II: MICROSEPARATION TECHNIQUES AND SAMPLE PREPARATION
Microseparation Techniques I: High Resolution Gel-Electrophoretic Techniques -
Qualitative, Quantitative and Micropreparative Applications
Microseparation Techniques II: Gel Electrophoresis for Sample Preparation in Protein Chemistry
Microseparation Techniques III: Electroblotting
Microseparation Techniques IV: Analysis of Peptides and Proteins by Capillary Electrophoresis
Microseparation Techniques V: High Performance Liquid Chromatography
Sample Preparation I: Removal of Salts and Detergents
Sample Preparation II: Chemical and Enzymatic Fragmentation of Proteins
SECTION III: BIOANALYTICAL CHARACTERIZATION
Amino Acid Analysis
Chemical Methods for Protein Sequence Analysis
Analyzing Post-Translational Protein Modifications
Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
MALDI Postsource Decay Analysis
Electrospray Mass Spectrometry
Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS)
Sequence Analysis of Proteins and Peptides by Mass Spectrometry
SECTION IV: COMPUTER SEQUENCE ANALYSIS
Internet Resources for Protein Identification and Characterization
Protein Sequences and Genome Databases
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