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Veterinary Technician's Handbook of Laboratory Procedures

December 2013, ©2014, Wiley-Blackwell
Veterinary Technician

Description

Veterinary Technician's Handbook of Laboratory Procedures is a quick-reference guide to conducting common laboratory tests. Carefully designed for fast access, the book covers common laboratory equipment, quality control, blood analysis, urinalysis, parasitology, and cytology. Important information is clearly and succinctly outlined for frequently used lab tests, providing step-by-step procedures, discussions of common errors, and tips and tricks, with more than 200 color images to aid in identification.

A companion website offers case studies, crossword puzzles, figures from the book in PowerPoint, and additional figures not found in the printed book at www.wiley.com/go/bellwoodhandbook. Veterinary Technician's Handbook of Laboratory Procedures is an invaluable tool for finding essential information on performing a wide range of laboratory tests.

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Table of Contents

Preface xi

Acknowledgments xiii

About the Companion Website xv

1 Laboratory Equipment 3

Laboratory equipment 3

Microscope 3

Centrifuge 9

Refractometer 11

Chemistry analyzers 13

Hematology analyzers 13

Coagulation analyzers 14

Quality control of all laboratory equipment 14

2 Blood Analysis 17

Hematology 17

Proper blood collection and handling techniques 17

Blood collection tubes 17

Peripheral blood smears 18

Staining 22

Packed cell volume (PCV) 23

Red blood cell count 26

Red blood cell indices 28

Reticulocyte count 29

Corrected reticulocyte percentage 30

Reticulocyte production index (RPI) 31

White blood cell count 31

White blood cell differential 33

Calculations 34

White blood cell estimate 35

Corrected white blood cell count 36

Platelet count 37

Platelet estimate 38

Normal species variation of leukocytes 40

Neutrophil maturation 44

Abnormal morphological changes in leukocytes 45

Erythrocyte morphologies 46

Evaluating a blood film to complement automated analyzers 51

Clinical chemistries 52

Coagulation testing 53

Activated clotting time 54

Buccal mucosal bleeding time 54

Automated clotting tests 55

3 Urinalysis 57

Sample collection 57

Free catch (voided sample) 57

Bladder expression 57

Catheterization 58

Cystocentesis 59

Sample preservation 59

In vitro changes occurring in unpreserved urine 60

Refrigeration 60

Freezing 61

Toluene 61

Formalin (40%) 61

Boric acid (0.8%) 61

Commercial preservation tablets 61

Physical examination of urine 62

Volume 62

Color 62

Turbidity 62

Odor 63

Specific gravity (SG) 64

Chemical examination 65

Glucose 66

Bilirubin 66

Ketones 67

Blood 67

pH 67

Protein 67

Urobilinogen 67

Nitrite 68

Leukocytes 68

Sediment examination 68

Materials required 68

Sediment stain 68

Procedure for microscopic examination of urine 69

Reporting of findings 70

Microscopic elements of urine sediment 70

4 Parasitology 77

Collection 77

Pooled samples 77

Gross examination 78

Consistency 78

Color 78

Blood 78

Mucous 78

Gross parasites 78

Microscopic examination 79

Direct fecal smear 79

Simple fecal fl otation 80

Centrifugation technique 81

Baermann technique 83

Common parasites of domestic species 83

Canine and feline 83

Ruminants 106

Equine 113

5 Cytology 121

Sample collection 121

Swabs 121

Fine needle biopsy 123

Tissue biopsy 125

Imprints 127

Centesis 128

Scrapings 129

Smear preparation 130

Feather smear method 130

Line smear method 131

Compression (or squash) method 131

Modified squash method 133

Starfish method 133

Staining 135

Romanowsky stains (Wright’s and Diff-Quick) 135

New methylene blue 135

Microscopic evaluation of cytology slides 136

Pyknosis and karyorrhexis 136

Karyolysis 138

Classifying cell types 138

Inflammation 138

Other cell types 140

Neoplasia 140

Reporting your findings 144

Laboratory submissions 144

Ear cytology 144

Slide preparation and staining 145

Microscopic findings 145

Vaginal cytology 145

Collection methods 147

Cell types seen 148

Stages of estrous 152

Semen evaluation 154

Slide preparation 154

Live/dead sperm ratio 155

Sperm morphology 155

Appendix 159

Temperature conversions 159

Metric conversions 159

Length 159

Weight 160

Volume 160

Metric system 160

Glossary 161

Bibliography 169

Index 171

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Author Information

Brianne Bellwood, RAHT, is a full time instructor and the Program Coordinator for the Animal Health Technology program at Lakeland College in Vermilion, Alberta, Canada. She covers both the practical and theoretical components of several laboratory subjects for Animal Health Technology students. She is also the Program Coordinator for the Animal Health Technology program at Lakeland College.

Melissa Andrasik-Catton, RVT, BS, was a full time instructor for 10 years and is now an adjunct instructor for Maple Woods Community College Veterinary Technology Program.  She is also a Clinical Trials Manager for Product Development and Regulatory Affairs at Dechra, Development, LLC.  She frequently lectures on the subject of Clinical Pathology at local, state and regional veterinary conferences.

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The Wiley Advantage

  • Provides a practical bench-top reference to essential information on conducting laboratory tests in veterinary practice
  • Puts all the information needed at your fingertips
  • Covers equipment, blood analysis, urinalysis, parasitology, and cytology
  • Designed for quick reference in the clinic or school setting
  • Presents more than 200 color images as a tool for identifying unfamiliar structures
  • Includes access to a companion website offering case studies, crossword puzzles, figures from the book in PowerPoint, additional images not found in the book, and an ear mite video at www.wiley.com/go/bellwoodhandbook
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Reviews

“Overall, this is a moderately priced and useful quick reference book.”  (Journal of the American Veterinary Medical Association, 15 June 2014)

“A 'must' for any practicing technician's reference library.”  (Midwest Book Review, 1 July 2014)

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Purchase Options
E-book   
Veterinary Technician's Handbook of Laboratory Procedures
ISBN : 978-1-118-72604-4
200 pages
September 2013
$41.99   BUY

Paperback   
Veterinary Technician's Handbook of Laboratory Procedures
ISBN : 978-1-118-34193-3
200 pages
December 2013
$51.99   BUY

Wiley E-Text   
Veterinary Technician's Handbook of Laboratory Procedures
ISBN : 978-1-118-73937-2
200 pages
October 2013
$51.99   BUY

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