The Journal of Eukaryotic Microbiology

Published on behalf of the International Society of Protistologists

Edited by:
Denis Lynn

Print ISSN: 1066-5234
Online ISSN: 1550-7408
Frequency: Bi-monthly
Current Volume: 57 / 2010
ISI Journal Citation Reports® Ranking: 2008: 66/91 Microbiology
Impact Factor: 1.502

TopAuthor Guidelines

GENERAL POLICIES AND GUIDELINES

(Revised guidelines as of August 2009, superseding all earlier versions)

The Journal of Eukaryotic Microbiology is copublished by Wiley-Blackwell and the International Society of Protistologists. Six issues are published per year on original research on protists, including lower algae and fungi, and covering all aspects of such organisms (e.g. behavior, biochemistry, cell biology, chemotherapy, development, ecology, evolution, genetics, molecular biology, morphogenetics, parasitology, physiology, systematic, and ultrastructure).

NEW: The Journal of Eukaryotic Microbiology is covered by Wiley-Blackwell Publishing's Early View service. Early View articles are complete full-text articles published online in advance of their publication in a printed issue. Articles are therefore available as soon as they are ready, rather than having to wait for the next scheduled print issue. Early View articles are complete and final. They have been fully reviewed, revised and edited for publication, and the authors' final corrections have been incorporated. Because they are in final form, no changes can be made after online publication. The nature of Early View articles means that they do not yet have volume, issue or page numbers, so Early View articles cannot be cited in the traditional way. They are therefore given a Digital Object Identifier (DOI), which allows the article to be cited and tracked before it is allocated to an issue. After print publication, the DOI remains valid and can continue to be used to cite and access the article.

1. The Journal publishes Regular Articles, Short Communications, Invited Reviews, and Supplements. Articles must be submitted in English only and prepared following the CHECKLIST FOR MANUSCRIPT PREPARATION (see below). Abstracts of annual meetings of the International Society of Protistologists and its Sections are accessible through The Journal Website: www.blackwellpublishing.com/journal.asp. Special reports resulting from workshops are published as Supplements following the approval of the Execute Committee of the Society.

2. Page charges. Authors are assessed page charges of $50.00 (U.S.) per black and white page and $900.00 (U.S.) per color page. Regular, student, honorary, and emeritus members of The Society will receive up to six (6) free black and white pages per volume (applied to one paper). There are no page charges to authors for a symposium article, past-president's address, invited review, book review or in memoriam article. Society members submitting abstracts are entitled to one free submission each year. Charges for reproductions of Color Plates must be negotiated with the Editorial Office of The Journal prior to acceptance. Young investigators may request support for page charges and color reproductions from the International Society of Protistologists' R. F. Hall Fund. Address requests to the Editor-in-Chief at editor@jeukmic.com. Support will be awarded to those authors whose papers are found meritorious upon review.

3. Accepted Manuscripts. All correspondence with authors of manuscripts accepted for publication will be carried on with the Editorial Office. Text and Figure Proofs must be returned to Wiley-Blackwell by email within 48 hours of their receipt, with an accompanying LIST of corrections. Delays in the return of proofs can lead to delays in publication. The responsibility for the accuracy of the article lies with the authors. The Publisher may proceed with publication if no response is received in a timely fashion. Due to the high cost of corrections in proofs, no substantial alterations can be permitted after page proofs have been generated. Do not make changes that affect matters of style only.

4. Reprints. Each author is entitled to 1 free PDF of their article. For additional offprints at the author's expense, access the Order Form Site by linking through your proof notification email and return it with the correct remittance to : Chris Jones at Sheridan Reprints (FAX: 717-633-8929). Reprints cannot be ordered through the Editorial Office.

5. Errata. Substantive retractions and corrections will be published after approval by all authors of the paper and will be identified in the Table of Contents.

6. Classification systems. Contributors are urged to become familiar with the systems of classification of the protists. See Adl et al., 2005, J. Eukaryot. Microbiol., 52:399-451 and Lee et al., 2002, An Illustrated Guide to the Protozoa, 2nd ed., the Society of Protozoologists, Lawrence, KS. Authors are not required to adopt the names or the scheme of classification used/proposed therein but, if using or proposing different suprafamilial ranks or names (including spellings), it would be advisable to provide the rationale for such changes.

7. Subspecies and strain designations. Sources of strains (e.g. American Type Culture Collection, Culture Centre for Algae and Protozoa, etc.) with catalogue or accession numbers are to be acknowledged in the MATERIALS AND METHODS section or given in appropriate tables. If not obtained from a recognized reference collection, indicate from whom and where the strain was obtained and provide a history (e.g. obtained from Ching Kung, University of Wisconsin, who isolated the mutant).

8. New Taxa. In descriptions of new taxa, use the standard abbreviations after the proposed name (generally in the title, abstract, and following at least its first usage in the Results, Discussion, and all figure explanations), as appropriate (n. sp., n. g., n. fam., n. ord., n. cl., etc.).

Authors are asked to write the complete description of the new taxon in the Results Section, using complete sentences and referring carefully to figures, as appropriate, to explain the characters of the new taxon. In the Discussion Section, the justification should be presented with reference to the published literature so that a careful rationale is provided for the new taxon. This text should then be followed by some kind of concluding sentence that leads into the formal Diagnosis. This should begin with a listing of the higher taxon to which the new taxon belongs, as centered text with authorship of the taxa. Please refer to recent issues of The Journal for style. Following this should be the formal taxonomic presentation to include, as appropriate, the following sections: Diagnosis, Dedication, Etymology including gender, Type Locality, Type Definitive Host, Type Intermediate Host, Prevalence, Ecology/Habitat, Deposition of Type Specimens and Materials, and other sections if needed.

Authors of papers on new taxa must deposit a fixed specimen in a recognized repository and provide an accession number in the manuscript. The following will accept fixed specimens within the USA: (1) U. S. National Parasite Collection, Dr. Eric P. Hoberg, Chief Curator, USDA, ARS, BARC-East No. 1180, 10300 Baltimore Ave., Beltsville, Maryland 20705, Telephone (301) 504-8588; FAX: (301) 504-8979; e-mail: ehoberg@anri.barc.usda.gov; (2) International Protozoan Type Slide Collection, Dr. Klaus Ruetzler, Curator (contact: Linda Cole), Smithsonian Institution, P.O. Box 37012, National Museum of Natural History, MRC-163, Washington, D.C. 20013-7012, Telephone (202) 633-1763; e-mail: Cole@si.edu; and (3), Dr. Scott Lyell Gardner, Curator of Parasites and Director of the Harold W. Manter Laboratory of Parasitology, W-529 Nebraska Hall, University of Nebraska, Lincoln, Nebraska 68588-0514, Telephone: (402) 472-3334; FAX: (402) 472-8949; Website: http://hwml.unl.edu. For apicomplexans, see also Bandoni & Duszynski, 1988, J. Parasitol., 74:519--523.

Other repositories for deposition of typeslides:

Dr. Erna Aescht, Biologiezentrum/Oberoesterrichische Landesmuseen,
J.-W.-Klein-Str. 73, 4040 Linz/Dornach, Austria
Tel.: (0043) - (0) 732-759733-53
FAX: (0043) - (0) 732-759733-99
URL: http://www.biologiezentrum.at

For cases in which there are living cultures it is suggested that the strain be deposited in a recognized reference collection. Contact Dr. Robert Molestina, American Type Culture Collection, Protistology Collection, 10801 University Boulevard, Manassas, Virginia 20110-2209 USA, telephone (703) 365-2700, ext. 2628 (lab ext. 2291) email: molestina@attc.org OR Dr. John G. Day, Curator CCAP, Culture Collection of Algae and Protozoa, Scottish Association of Marine Science, Dunstaffnage Marine Laboratory, Dunbeg Argyll, PA37 1QA, UK: telephone (44) 1631 559000; direct dial (44) 1631 559349; FAX: (44) 1631 559001; email: www.ccap.ac.uk .

Please note: The Editors consider it professionally ethical to share new strains, mutant strains, hybridomas, plasmid DNA, and biological reagents used in reported work. Hence, authors who submit to The Journal of Eukaryotic Microbiology are expected to provide others with relevant material, at reasonable cost, when requested.

9. Sequences of Nucleotides and Proteins. New sequences must be deposited in a database accessible to the public, such as GenBank or EMBL. Accession numbers must be included in the final version of the accepted manuscript. Sequence alignment data can also be submitted to EMBL.

Authors must convince the Associate Editors and Reviewers that methods have been followed that ensure the production of the highest quality and accuracy of sequence data. For DNA sequences, this could involve sequencing both strands completely or sequencing a single strand from at least two independent PCR reactions.

Associate Editors and Reviewers may occasionally request sequences or alignments from authors to verify claimed results. Authors must be prepared to provide these data as an e-mail attachment.

Editors consider it professionally ethical to share alignment files. Hence, authors who submit to The Journal of Eukaryotic Microbiology are expected to provide others with these data, when requested.

SUBMISSION OF MANUSCRIPTS

Manuscripts must be submitted on-line at http://mc.manuscriptcentral.com/jeukmic/. If authors are unable to do this, please contact the Editorial Office at editor@jeukmic.com.

Wiley-Blackwell Author Checklist available at: http://www.blackwellpublishing.com/authors/checklist.asp

The Journal of Eukaryotic Microbiology

CHECKLIST FOR MANUSCRIPT PREPARATION

(JEU checklist revised as of August 2009, superseding all earlier versions)

(Check Each Item Prior to Submission of Manuscript)

Follow instructions exactly to prevent delays in review and handling of your manuscript.

I. General Instructions

[ ] Articles must be submitted in English. British spelling is permitted, but must be used consistently.

[ ] Use 8.5 x 11 inch paper (21.59 x 27.94 cm). Leave a 1-inch (2.54 cm) margin on all sides. DO NOT use proportional spacing.

[ ] DOUBLE SPACE EVERY SECTION OF THE MANUSCRIPT, INCLUDING TABLES, LEGENDS, AND LITERATURE CITED.

[ ] DO NOT alter the margins to indent text. Indent only by typing one tab character. DO NOT use multiple spaces to indent. DO NOT use tab characters for any other purpose.

[ ] DO NOT use line justification or hyphenation. DO NOT use page breaks. DO NOT use columns.

[ ] DO NOT use hard returns, except at the ends of paragraphs and after section headings. DO NOT add extra lines between paragraphs or sections, after heads or titles.

[ ] Bold, italics, underline, superscript, and subscript are the ONLY formatting commands that may be used. Rather than underline text, use word processor functions to make text bold and italic.

[ ] Use a 12-point font that distinguishes the letter 'el' from the number one (i.e. do not use Helvetica).

[ ] Number pages consecutively.

[ ] Assemble manuscript in this order: 1) Title page, including footnotes, 2) Abstract page, 3) Text, 4) Literature Cited, 5) Figure legends, 6) Tables. Figures, (please see below).

Abbreviations and technical expressions:

[ ] All chemical and mathematical terms, formulae, abbreviations, acronyms, and symbols should follow the style used by The Journal of Biological Chemistry (www.jbc.org). For other style questions, consult the sixth edition of the Council of Biology Editors Manual of Scientific Style and Format or contact the Editorial Office.

[ ] Use the metric system only. Use these abbreviations: g (gram), ml, h, min, s, yr, mo, wk, diam., SD, (Spell out liter.). Use g (italicized) not rpm for centrifuge information.

[ ] Spell out numbers and abbreviations at the beginning of sentences.

[ ] All abbreviations and acronyms are placed in parentheses following the names written out in full the first time they are mentioned in the text.

[ ] DO NOT italicize common Latin words, abbreviations or phrases such as 'et al.', 'e. g.' or 'in vivo'. DO italicize the names of genes (e. g. Arpl gene, Arpl mRNA). DO NOT italicize the names of proteins (e. g. Arpl protein, antibody raised against Arpl).

[ ] Leave a space between mathematical symbols and numbers (i.e. 5 + 4 and 6

[ ] Hyphenate measurements when used as a compound adjective (e. g. a 10-ml tube, a 45-kb sequence).

[ ] Use 1,000 not 1000. Use 0.13 not .13. Use 13% not 13 percent. Use 12 °C not 12°C nor 12° C.

[ ] Write out numbers 'one' to 'nine,' UNLESS used as a measurement or in an equation (e.g. four organisms, 4 ml, 4 + 5).

[ ] Type two hyphens between RANGES of numbers, such as page numbers and measurements (i. e. 41:123--189 and about 6--7 min).

II. Title Page

[ ] Running Head. Authors' last names and a short title in uppercase letters separated by three hyphens (see below). Maximum length 60 characters including spaces.

SMITH & JONES---SHORT TITLE

SMITH ET AL.---SHORT TITLE

[ ] Title. Capitalize first letter of each word, except articles, prepositions, and conjunctions. In bold.

[ ] Authors' names. First name, middle initial, last name. Capital letters, all bold. Use superscript lowercase, not bold, letters, (a, b, c) after names to denote affiliations. Use superscript numbers (1, 2, 3) after names to denote footnotes for current address information.

SALLY R. SMITHa,1 and JOHN B. JONES b

[ ] Authors' addresses: List in order of affiliation, preceded by lower case letter in superscript. Addresses, in italics, may include street addresses and PO boxes if necessary. Write out state names.

aResearch Lab, School of Science, City, State Postal Code, State, Country

[ ] Corresponding Author: In this unnumbered footnote include the first initial and last name of the corresponding author, complete street address, followed by telephone number, FAX number, and e-mail address. Please note that it is very important that the corresponding author's email address is correct since proofs will be sent to the email address provided. Example as follows:

Corresponding Author: D. Lynn, Dept. of Integrative Biology, University of Guelph, 50 Stone Road W., Guelph, ON N1G 2W1 Canada---Telephone number: 519-826-5724; FAX number: 519-826-5725; e-mail: editor@jeukmic.com

[ ] Footnotes: Below the Corresponding Author footnote, include additional un-numbered and all numbered footnotes.

III. Abstract Page (Ms page 2)

[ ] Begin with the word 'ABSTRACT.', followed by the first sentence. DO NOT indent. Write one paragraph of 200 words or less.

[ ] Include brief statements of purpose, methods, results, and significance. DO NOT cite any references. DO NOT use any abbreviations or acronyms.

[ ] Key Words. Following the abstract, list no more than 10 key words or phrases that are NOT already in the title. Tab indent 'Key Words' in bold. Then, list, in alphabetical order, but not in bold, words or phrases separated by commas. End with a period, as follows:

Key Words. Brachiola algerae, human isolate, infection, insect isolate, in vitro culture, microsporidia, spore germination.

IV. Text (begin on Ms page 3)

[ ] Begin with a brief introduction. DO NOT tab indent first paragraph. DO NOT use a heading such as 'Introduction'.

[ ] Headings: Use the following headings, in this order: MATERIALS AND METHODS, RESULTS, DISCUSSION, and ACKNOWLEDGMENTS. Do not combine RESULTS and DISCUSSION unless you are submitting a Short Note. These headings are all capitals, not bold. Double space between these sections of the text.

[ ] Subheadings: Tab indented, in bold, end with a period. First word begins with a capital letter. Text follows immediately on same line.

[ ] Lower subheadings: Tab indented, in italics, end with a period. First word begins with a capital letter. Text follows immediately on the same line.

[ ] Cite each figure and each table in numerical order in the text.

[ ] Use 'Fig.' (not Figs. or fig.) throughout the manuscript (i.e. Fig. 3, Fig. 3--5, Fig. 2, 3). To cite tables and figures together, use (Table 1 and Fig. 2, 3).

[ ] Check that every text citation is referenced in the Literature Cited and that each reference in the Literature Cited has been included in the text. Published references used in legends and figures must also be included in the Literature Cited.

[ ] Strain/subspecies information must be included under MATERIALS AND METHODS. See General Policies, item #7.

[ ] For reagents and equipment necessary to repeat the procedures described in the MATERIALS AND METHODS section, Please provide (in parentheses) the company/supplier name, city, and state or country, upon first mention. If abbreviated, spell out the company/supplier at first mention.

Literature citations within the text are as follows:

[ ] Published and in press citations are referenced in parentheses by author and year. Authors and year are NOT separated by commas.

[ ] Text citations of two or more works must be placed in alphabetical order separated by semicolons. More than one work by the same author(s) in the same year must be identified by the letters a, b, c, after the year of publication. Use: (Fenchel 1987; Smith and Jones 1984a, b). (NOTE: In revisions double check that the a, b, c, are unchanged).

[ ] For works with MORE THAN TWO AUTHORS use et al.

for example: (Smith et al. 1986).

[ ] Unpublished works/data in the text are cited in parentheses as follows:

For an author: (SS., unpubl. data) or (SRS., pers. observ.).

For other sources: (Smith, S. R., unpubl. data) or (Smith, S. R., pers. commun.).

[ ] Manuscripts in preparation and those submitted but not yet accepted must be cited as unpublished data.

[ ] For journals (e.g. BMC, PLoS) where article number is referenced rather than pagination:

Hampl,V., Silberman, J. D., Stechmann, A., Diaz-Trivin'o, S., Johnson, P. & Roger, A. J.
2008. Genetic evidence for a mitochondriate ancestry Q5 in the 'amitochondriate' flagellate Trimastix pyriformis. PLoS ONE, 3, art. 1383.

V. Acknowledgments

[ ] ACKNOWLEDGMENTS are arranged in paragraph form as brief comments. The editors consider it professionally ethical to consult individuals before they are acknowledged for their assistance.

VI. Literature Cited

[ ] Continue double spacing. Use hanging indents.

[ ] Articles in preparation or submitted are NOT listed in the LITERATURE CITED section. Cite these in the text. See above.

[ ] Begin with the heading: LITERATURE CITED, all capitals.

[ ] Arrange references alphabetically by last name of first author. References by a single author precede multi-authored works by the same senior author, REGARDLESS of date. List works by the same set of authors chronologically, beginning with the earliest. See below:

Smith, S. R. 1994.

Smith, S. R. & Jones, J. B. 1984a.

Smith, S. R. & Jones, J. B. 1984b.

Smith, S. R. & Jones, J. B. 1993.

Smith, S. R. & Sanford, Y. M. 1988.

Smith, S. R., Jones, J. B. & Miller, P. K. 1992.

Smith, S. R., Jones, J. B. & Zimmer, L. M. 1989.

Smith, S. R., Jones, J. B., Miller, P. K. & Zimmer, L. M. 1986.

[ ] List every author's name for every reference. DO NOT use et al. or a long dash.

[ ] Format references as shown below:

JOURNAL ARTICLES:

Oh, S. W. & Jeon, K. W. 1998. Characterization of myosin heavy chain and its gene in Amoeba proteus. J. Eukaryot. Microbiol., 45:600--605.

ARTICLES IN BOOKS:

Van Houten, J., Hauser, D. C. R. & Levandowsky, M. 1981. Chemosensory behavior in protozoa. In: Levandowsky, M. & Hutner, S. H. (ed.), Biochemistry and Physiology of Protozoa. 2nd ed. Academic Press, New York. 4:67--124.

BOOKS:

Fenchel, T. 1987. Ecology of Protozoa. The Biology of Free-Living Phagotrophic Protists. Science Tech Publishers, Madison, Wisconsin. p. 53--57.

THESES:

Vacchiano, E. 1992. The establishment of Vorticella as a model system for the study of calfilamins. Dissertation. University of Illinois at Chicago, Chicago, IL. 155 p. Available on microfilm from University of Michigan, Accession Number AAG9310158.

ABSTRACTS:

Hinkle, G. & Sogin, M. L. 1998. Preliminary genomic analysis of the microsporidian parasite Spraguea lophii. Abstract 2. J. Eukaryot. Microbiol., 45:1A.

[ ] Use SPACES between author initials. For multi-authored works, use '&' for 'and.'

[ ] Journal names should be italicized and abbreviated (except for one-word titles) following a documented source, e.g. the Serial Sources for the BIOSIS Previews Database, published annually by BIOSIS, 2100 Arch St., Philadelphia, PA 19103-1399 USA or Online Journal Search Biosis Previews.

[ ] The volume number is in BOLD but NOT the colon following. Page numbers are flush with the colon; not bold. Place 2 hyphens between page numbers.

[ ] 'In Press' citations must have been accepted for publication and the name of the journal or publisher included.

[ ] Verify all entries against original sources, especially journal titles, accent and diacritical marks, and spelling in languages other than English. Capitalize all nouns in the citation title in German. Authors bear responsibility for accuracy.

VII. Figure Legends (continue ms page numbering)

[ ] Start figure legends on a new page. Use paragraph form (i.e. tab indent) and double space the text beginning with 'Fig'. Figure numbers only are in bold.

[ ] Figure captions should be brief and self-explanatory. Species names are italicized and spelled out in full the first time they are used in each caption. For plates with more than one figure, a first sentence or phrase should summarize all figures on the plate. Then describe each figure on the plate individually.

Fig. 1--4. Life cycle of Gregarina coronata n. sp. 1. Trophozoites. 2. Immature gamonts. 3. Mature gamonts. 4. Oocysts.

[ ] DO NOT use a new page for each legend.

[ ] Symbols appearing in a figure must be explained in the legend, and used preferably in the text of the legend. List other symbols alphabetically, like this: C, cell; N, nucleus.

VIII. Tables (continue ms page numbering)

[ ] Use a separate WORD document for each table. Double space everything. Assign each table an Arabic number.

[ ] DO NOT reduce type size anywhere in tables, including superscripts and subscripts.

[ ] The table caption should be brief and self-explanatory. Species names are italicized and spelled out in full the first time they are used in the caption. Use phrases or sentences typed above the table as shown below (only the table number is in bold).

Table 1. Lipid composition of cell membranes of Pneumocystis carinii.

[ ] Indicate footnotes with lower case superscript letters, like thisa, and like thisb. DO NOT reduce the type size. The footnotes themselves should be typed under the table, like this:

aSome experiments conducted at 4 °C.

[ ] Use asterisks to indicate statistical significance.

[ ] DO NOT use any vertical lines.

IX. Figures

Please refer to the following webpage for figure submission guidelines: http://www.blackwellpublishing.com/bauthor/illustration.asp

Please be sure to check dpi before final submission.

[ ] Illustrations may be black and white or color photographs, halftones, line drawings or graphs.

[ ] Arrange related figures to form plates. Figures must be arranged in the order in which they are cited in the text. They should be assigned Arabic (1, 2, 3) numbers.

[ ] DO NOT combine line drawings with halftones. Arrange them on separate plates.

[ ] Figures of gels must include a high-quality photograph of the gel and be labelled with high quality lettering.

[ ] Maximum figure width following reduction: one column = 89 mm, full = 181 mm. Maximum height = 231 mm.

[ ] To conserve space, figures will be reduced to one column width (89 mm) whenever practical. Please ensure that capital letters and numbers are not less than 2 mm high after reduction. Authors will be required to redo figures if they do not meet these standards or will be charged if the Printer must do the revisions.

[ ] DO NOT combine color figures with any other type of figure. Each plate must be printed either in black and white or color. (Color figures can be placed on plates with black and white figures if the whole plate is to be printed black and white.) Indicate at manuscript submission, which figures are to be printed in color. If no notice is given, all figures will be printed in black and white. Try to place all color figures on one plate. There is a considerable extra charge for color reproductions. Authors are responsible for these charges. Contact the Editorial Office for information about submitting color figures

Figure preparation:

[ ] Crop unnecessary area from each figure.

[ ] Individual figures of a plate should be butted together but must be separated by white demarcation lines. Do leave a 2.54 cm margin on each side of the plate.

[ ] Label individual figures on plates with an Arabic number in the LOWER RIGHTHAND CORNER. DO NOT use letters. Figures to be printed individually should be numbered in the legend only.

[ ] Add labels and symbols to figures, preferably Helvetica or Franklin Gothic. Labels must be at least 2 mm tall following reduction.

[ ] Photomicrographs must include scale bars. Define the bar in the legend (e.g. Scale bar = 4 µm).

[ ] Figures must be prepared to professional standards.

For production purposes, authors will be asked upon acceptance of their papers to submit:

--Manuscript text and each table submitted separately as WORD files,

--Line artwork (vector graphics) in EPS format with a resolution of at last 600 dpi at final size.

--Bitmap files (halftones or photographic images) in TIFF format with a resolution of at least 600 dpi at final size. Detailed information on submitting electronic artwork can be found at http://www.blackwellpublishing.com/authors/digill.asp

Line drawing and graph preparation:

[ ] DO NOT place 'legends' within a graph or figure. This information must be included in a regular figure legend at the end of the text file.

[ ] Labels on axes should begin with a capital letter, centered on the length of the axis. Units of measurement follow in parentheses. Multiplication factors are associated with the axis label not with the unit of measurement.

[ ] Line drawings may be reduced by the editor. Be sure to use symbols and labels that are large enough to be at least 2 mm tall after reduction.

Sequence data:

[ ] Prepare sequence data as a figure with an appropriate legend.

[ ] DO NOT prepare a figure for a single sequence, but refer to the Accession Number in the appropriate database.

[ ] Sequence alignments should be edited to exclude long sections of identical positions.

[ ] Sequence original width must be 180 mm. Sequence original height must be

[ ] Each letter must be 2.6--4 mm tall. Use Courier 16-point for mostly small letter, and Courier 14-point for mostly capital letter sequences.

Note: The final width of the figure will be 140 mm. (The sequence will be centered on the page.) The final height of each individual character should be no less than 2 mm and no more than 3 mm.

Preparation of final draft:

Note: Be sure to proofread your manuscript carefully. It is a major responsibility of the author(s) to insure that the manuscript is written clearly and is grammatically correct.

[ ] Please ensure that each figure, each table, and each reference is cited in the text. Check this after revision of the manuscript.

XI. Short Communications

[ ] A Short Communication must not exceed 1,500 words of text. If it includes one figure and one table, the limit is 1,200 words. Style and format are identical to that of full-length papers except that RESULTS AND DISCUSSION are placed together. Papers must be complete in themselves, presenting sufficient information to allow repetition of the results. The abstract should be 100 words long.

XII. Cover Letter

[ ] Include a statement that the manuscript contains only original data that have not been submitted or published elsewhere. With the exception of invited reviews, The Journal of Eukaryotic Microbiology will not publish articles that contain substantial data that have been previously published or submitted.

[ ] State which author should be billed for the page charges. If no author is named, the corresponding author will be invoiced.

[ ] If any significant changes were made during revision of the manuscript, list these manuscript changes in detail in the cover letter or in a separate attachment.

XIII. What and Where to Submit

[ ] Submit the following files:

1. One cover letter (see XII).

2. The manuscript file in WORD format.

3. The tables, in WORD format, each submitted as a separate file.

4. The figures with resolution of at least 600 dpi in Encapsulated PostScript (eps) or Tag Image File (tif) formats, each plate submitted as a separate file.

[ ] On-line submissions are made to :

http://mc.manuscriptcentral.com/jeukmic/

If problems are encountered during submission, please use the 'Help' link at the ScholarOne Manuscripts website; if necessary, contact ScholarOne Support<<a href='mailto:chuck.pistole@Thomson.com'>chuck.pistole@Thomson.com> or the Editorial Office e-mail (editor@jeukmic.com).

[ ] Upon acceptance, a completed Exclusive Licensing Form (http://www.blackwellpublishing.com/pdf/JEU_ELF2006.PDF) must be returned to the Editorial Office. Please return the form as a scanned email attachment to editor@jeukmic.com. Failure to return the form will delay the publication of manuscripts.

[ ] OnlineOpen is available to authors of primary research articles who wish to make their article available to non-subscribers on publication, or whose funding agency requires grantees to archive the final version of their article. With OnlineOpen the author, the author's funding agency, or the author's institution pays a fee to ensure that the article is made available to non-subscribers upon publication via Wiley InterScience, as well as deposited in the funding agency's preferred archive. For the full list of terms and conditions, see http://www3.interscience.wiley.com/authorresources/onlineopen.html#OnlineOpen_Terms.

Any authors wishing to send their paper OnlineOpen will be required to complete the payment form available from our website at:

http://www3.interscience.wiley.com/authorresources/onlineopen.html#OnlineOpen_Terms.

Prior to acceptance there is no requirement to inform an Editorial Office that you intend to publish your paper OnlineOpen if you do not wish to. All OnlineOpen articles are treated in the same way as any other article. They go through the journal's standard peer-review process and will be accepted or rejected based on their own merit.