Wiley Cytometry Web Site

Journals Library Instruments Sites Home

Library: Wiley Flow Cytometry Titles

Flow Cytometry and Sorting, Second Edition

Affiliations
Myron R. Melamed, M.D.
Departments of Pathology
Memorial Sloan-Kettering Cancer Center and Cornell University Medical College
New York, New York

Tore Lindmo, Ph.D.
Biophysics Group
Division of Physics
University of Trondheim - NTH
Trondheim, Norway

Mortimer L. Mendelsohn, M.D., Ph.D.
Biomedical and Environmental Research
Lawrence Livermore National Laboratory
Livermore, California

ISBN 0-471-56235-1 | 1990 | 832 pages; cloth

"This book is destined to replace the previous edition as a major reference in any flow cytometry laboratory."
American Journal of Clinical Oncology

"The first edition is a classic, but do clear space on the shelf. The new edition is a long-awaited replacement."
Cytometry

"All articles together make up a unique synopsis which will be the most important flow cytometry book for the years to come....The book should be found on the book-shelves of every flow cytometry room."
Studia Biophysica

"Flow Cytometry and Sorting serves as an excellent resource book for all scientists using flow cytometry....Every laboratory engaged in flow cytometry should have at least one copy of Flow Cytometry and Sorting because one copy will likely be borrowed frequently."
Environmental and Molecular Mutagenesis

"...a superb attempt to provide the reader with a thorough review of flow cytometry and its potential applications."
Cancer Cells

During the past decade, expansion of the techniques and applications of flow cytometry has broadened into clinical application that includes immunohematology and tentative steps in the analysis of solid tumors. The increasing use of flow cytometry in the study of cell biology has created the need for a new edition of a classic text, Flow Cytometry and Sorting. The completely updated and revised Second Edition describes and evaluates -- in greater detail -- the most recent practical applications of flow cytometry technique to basic cellular biological investigation and clinical research on human neoplasms. Ideal for the experience researcher as well as the novice, this informative volume offers state-of-the-art reviews on all aspects of flow cytometry, including instrumentation; methodologies for cell preparation, staining, and examination of various cell types; analysis of measurements; and applications in cell biology, clinically-oriented investigation, and established clinical diagnostic purposes. Its detailed coverage of the latest techniques in the field makes Flow Cytometry and Sorting, Second Edition an indispensable source for researchers in cell biology and the clinical investigation of human neoplasms , as well as oncologists, hematologists, immunologists, urologists, and cancer chemotherapists.

Table of Contents

Contributors
Preface

Part 1. Introduction
1. An Historical Review of the Development of Flow Cytometers and Sorters
Myron R. Melamed, Paul F. Mullaney, and Howard M. Shapiro

2. Characteristics of Flow Cytometers
Harold B. Steen

Part 2. Cytophysical Methods
3. Hydrodynamic Properties of Flow Cytometry Instruments
Volker Kachel, Hugo Fellner-Feldegg, and Everhard Menke

4. Electrical Resistance Pulse Sizing: Coulter Sizing
Volker Kachel

5. Light Scattering and Cytometry
Gary C. Salzman, Shermila Brito Singham, Roger G. Johnston, and Craig F. Bohren

6. Slit-Scanning
Leon L. Wheeless, Jr.

7. Electronics and Signal Processing
R.D. Hiebert

8. Flow Sorters for Biological Cells
Tore Lindmo, Donald C. Peters, and Richard G. Sweet

9. Commercial Instruments
Phillip N. Dean

Part 3. Cell Preparation
10. Preparation of Cell/Nuclei Suspensions From Solid Tumors for Flow Cytometry
M.G. Pallavicini, I.W. Taylor, and L.L. Vindelov

11. Methods for Enrichment of Cellular Specimens for Flow Cytometry and Sorting
A. Tulp and J.G. Collard

Part 4. Cytochemical Methods
12. Fluorescent Probes for Cytometry
Alan S. Waggoner

13. Cytochemical Techniques for Multivariate Analysis of DNA and Other Cellular Constituents
Harry A. Crissman and John A. Steinkamp

14. Fluorescent Probes of DNA Microstructure and DNA Synthesis
Samuel A. Latt and Richard G. Langlois

15. Acridine Orange: A Versatile Probe of Nucleic Acids and Other Cell Constituents
Zbigniew Darzynkiewicz and Jan Kapuscinsky

16. Probing Nuclear Chromatin by Flow Cytometry
Zbigniew Darzynkiewicz

17. Immunofluorescence Techniques
Michael R. Loken

18. Ligand Binding, Endocytosis, and Processing
Robert F. Murphy

19. Microspheres as Immunoreagents for Cell Identification and Cell Fractionation
T. Lea, J.P. O'Connell, K. Nustad, S. Funderud, A. Berge, and A. Rembaum

20. Ultrasensitive Molecular-Level Flow Cytometry
James H. Jett, Richard A. Keller, John C. Martin, Dinh C. Nguyen, and George C. Saunders

Part 5. Analysis of Measurements
21. Standards and Controls in Flow Cytometry
Paul Karl Horan, Katharine A. Muirhead, and Sue E. Slezak

22. Data Processing
Phillip N. Dean

Part 6. Cell Biology
23. Quantitative Cell-Cycle Analysis
J.W. Gray, F. Dolbeare, and M.G. Pallavicini

24. Multiparameter Flow Cytometry in Studies of the Cell Cycle
Zbigniew Darzynkiewicz and Frank Traganos

25. Flow Karyotyping and Chromosome Sorting
J.W. Gray and L.S. Cram

26. Flow Cytometry and Sorting of Sperm and Male Germ Cells
Barton L. Gledhill, Donald P. Evenson, and Daniel Pinkel

27. Mutagenesis as Measured by Flow Cytometry and Cell Sorting
Ronald H. Jensen and James F. Leary

28. Applications of Flow Cytometry and Sorting to Molecular Genetics
M.A. Van Dilla, M.E. Kamarck, and M. Lalande

29. Flow Cytometric Studies of Microorganisms
Harald B. Steen

30. Virus-Cell Interactions Analyzed with Flow Cytometry
John M. Lehman and James W. Jacobberger

31. Application of Flow Cytometry and Sorting to Higher Plant Systems
Michael H. Fox and David W. Galbraith

32. Measurement and Intracellular Ionized Calcium and Membrane Potential
Peter S. Rabinovitch and Carl H. June

Part 7. Hematology and Immunology
33. Analysis and Sorting of Blood and Bone Marrow Cells
Jan W.M. Visser

34. Applications in Immunology and Lymphocyte Analysis
Kenneth A. Ault

Part 8. Applications in Oncology
35. Flow Cytometry of Leukemia
Michael Andreeff

36. Flow Cytometry of Lymphoma
Michael Andreeff

37. DNA Flow Cytometry of Human Solid Tumors
Martin N. Raber and Bart Barlogie

38. Flow Cytometry in Clinical Cytology
Myron R. Melamed and Lisa Staiano-Coico

39. Single- and Multiparameter Analysis of the Effects of Chemotherapeutic Agents on Cell Proliferation In Vitro
Frank Traganos