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The Norris Cotton Cancer Center
Flow Cytometry Laboratory and Department of Physiology
Dartmouth-Hitchcock Medical Center
Lebanon, New Hampshire
ISBN: 0-471-56095-2 | 1992 | 216 pages, paperback
"Alice Longobardi Givan has done all of us a great service, by explaining to anyone with a high school science background exactly how flow cytometers work... a well produced, intelligently written beginners guide to flow cytometry. Everyone who uses cell sorters as a major research tool would find this book useful."
Immunology Today
"Alice Givan has filled the gap in the market. She has provided a simple, readable account of flow cytometry and its applications... I would recommend that every laboratory with a flow cytometer should keep a copy of Alice's book on their shelf to be handed to every novice on their first entry into the laboratory."
Journal of Clinical Pathology
"With this book, the field of flow cytometry... has been given the introductory textbook it deserves.... Throughout the book, the reader's interest and appetite for knowledge are well catered for by the inviting language and good humor that shine through the text on every page."
Trends in Cell Biology
Geared for the nonspecialist, Flow Cytometry: First Principles presents a succinct, accessible, "user friendly" approach to exploring flow cytometry and its relevance to modern biology and biomedicine. Starting with the fundamental of instrumentation, data analysis, and interpretation of experimental and diagnostic results, the author provides a tutorial treatment of applications both in the laboratory and the clinic that will appeal to the novice and expert alike.
Using concrete examples to illustrate general concepts, this self-teaching guide builds the reader's technical understanding of the capabilities and limitations of modern analytical cytology. Students, research faculty, clinical specialists, and laboratory technologists will gain an appreciation of the range of experimental procedures and diagnostic measurements that are possible with today's flow cytometry instrumentation.
An outgrowth of years of lecture and laboratory workshop instruction by the author, Flow Cytometry: First Principles is designed for the needs of those in cell and molecular biology, immunology, oncology hematology, laboratory medicine, and other areas that rely upon the quantitative measurement and selective purification of cells and their constituents. This practical introduction will prove indispensable for the the nonspecialist who wishes to understand the power and the pitfalls of this technology -- toward a more critical interpretation of the literature as well as the effective design and implementation of laboratory protocols.
Table of Contents
Preface
Acknowledgments
1. The Past as Prologue
2. Setting the Scene
3. Instrumentation: Beyond the Black Box
The Optical Bench
The Fluid System
Electronics
Sorting
4. Information: Harnessing the Data
Data Storage
Data Analysis
5. Seeing the Light: Lasers, Fluorochromes, and Filters
General Theory
The Illuminating Beam
Fluorochromes
Lenses, Filters, and Mirrors
Compensation
6. Cells From Without: Lymphocytes and the Strategy of Gating
Lymphocytes
Staining for Surface Markers
Controls
Quantification
The Strategy of Gating
7. Cells From Within: DNA and Molecular Biology
Fluorochromes for DNA Analysis
Ploidy
Cell Cycle Analysis
Two-Color Analysis for DNA and Another Parameter
Chromosomes
Molecular Biology
Cell Death
8. Disease and Diagnosis: The Clinical Laboratory
The Hematology/Immunology Laboratory
Oncology/Pathology Laboratories
Transplantation
Comments
9. Out of the Mainstream: Research Frontiers
Functional Assays
The Aquatic Environment
Animal Development
Microbiology and Cell Microdroplets
10. Flowing On: The Flow Cytometry Laboratory of the Future
General References
Glossary
Figure Credits
Index