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Handbook of Flow Cytometry Methods

Associate Editors
Zbigniew Darzynkiewicz
New York Medical College
Valhalla, New York

Phillip Dean
Lawrence Livermore National Laboratories
Livermore, California

Lynn Dressler
University of Arizona
Tucson, Arizona

Hans Tanke
Sylvius Laboratorium
Leiden, The Netherlands

Leon Wheeless
University of Rochester
Rochester, New York

ISBN 0-471-59634-5 | 1993 | 246 pages, comb-bound

A Concise Laboratory Benchtop Manual

"This text is indispensable for the bookshelf of any flow cytometry laboratory."
Immunology Today

"All in all, this book is an indispensable addition to the book shelves of flow cytometry laboratories."
FEBS Letters

"If this handbook does not already reside near your cytometer, buy it.... This is an excellent resource designed to elevate our collective scientific standards."
Cytometry

Drawing on their collective experience at leading research centers, the editors have fashioned a unique "working manual" intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry providing practical, step-by-step procedures for:

• The preparation and handling of standards, reagents and specimens
• Laboratory analyses for immunophenotyping
• Measurement of DNA content and cell cycle analyses
• Determination of cell physiology and function
• Set up and operation of flow cytometers
• Computational and data analysis techniques
• Safety precautions

Each method is described in detail to be used and modified depending on the user's needs. Special features of Handbook of Flow Cytometry Methods include:

• Ample space throughout for personal annotations
• Suggestions of alternative procedures; trouble-shooting tips
• Extensive list of reagents and suppliers
• Comb-bound format for ease of use at the lab bench

Table of Contents

Section Heads
Standards, Reagents, & Instruments
Precautions
Methods
Data Analysis & Computers
References Cited
Index of Terms