 ISAC Meeting Program and Abstracts Archive |
| Wiley Cytometry Web Site - ISAC 2000 International Congress |
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ISAC XX International Congress Program - Plenary and Parallel Session Abstracts - Go to Poster Abstracts |
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Friday, May 19, 2000 |
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6:00 8:00 |
Registration |
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Saturday, May 20, 2000 |
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7:00 5:30 |
Registration |
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8:00 10:00 |
Scientific Tutorial I. Flow Analysis: Principles and Instruments/Highspeed Sorting in Theory and Practice Scientific Tutorial II. Cell Cycle Proteins |
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10:00 10:30 |
Coffee Break |
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10:30 12:30 |
Scientific Tutorial III. Multicolor FISH and Comparative Genomic Hybridization Scientific Tutorial IV. Live Cell Cytometry: Intracellular Environments, Functional Assays, and Cell Signaling Scientific Tutorial V. Multiparameter DNA Measurement of Clinical Samples |
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12:30 1:30 |
Lunch on your own |
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1:30 3:30 |
Scientific Tutorial VI. High Density Array Technologies Scientific Tutorial VII. Cell Death Scientific Tutorial VIII. Multiparameter Flow Cytometry of Clinical Samples-Including 4-color Immunofluorescence |
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3:30 5:30 |
Scientific Tutorial IX. Genotyping of Single Nucleotide Polymorphisms Scientific Tutorial X. Practical Confocal Microscopy Scientific Tutorial XI. Bugs in the Beam-Cytometric Measurement of Microorganisms |
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5:30 7:30 |
Scientific Tutorial XII. Green Fluorescent Protein Scientific Tutorial XIII. Detection of Minimal Residual Disease Scientific Tutorial XIV. Cytometric Resources Dinner on your own |
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Sunday, May 21, 2000 |
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7:30 7:30 |
Registration |
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8:00 6:00 |
Exhibitor Set-up |
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8:00 9:00 |
Frontiers in Plant Biology |
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9:00 10:00 |
Frontiers in Marine Science |
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10:00 10:15 |
Break |
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10:15 - 11:15 |
Frontiers in Cell Cycle Regulation |
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11:15 1:00 |
Lunch on your own |
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1:00 2:00 |
Frontiers in Cytometry |
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2:00 3:00 |
Frontiers in Oncology |
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3:00 3:15 |
Break |
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3:15 5:15 |
PARALLEL SESSION I A Micro-electronic Flow Cytometer The Optical Sectioning Programmable Array Microscope (PAM) Three-Dimensional Arrangement of GFP-tagged Upstream Binding Factor (UBF) during the Cell Cycle of Living Cells The ACIS: Emerging Technology for Cellular Imaging The Violet Laser Diode as a Light Source for Cytometry HT-PS and Plug Flow Cytometry for Multi-Well Plate Drug Screening |
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Basic Cancer Biology Pancolonic Chromosomal Instability Precedes Dysplasia and Cancer in Ulcerative Colitis Nuclear Chromatin Texture Analysis of Histologically Normal Colorectal Mucosa in Patients with Adenomatous Polyps and Colorectal Cancer Interaction of Receptor Tyrosine-Kinases (RTK) of the EGFR-family and Related Growth Kinetics in Breast and Bladder Cancer Cells Heterogeneity of HLA Class I Expression in Cervical Cancer Detected by Multiparameter DNA Flow Cytometry Multidrug-resistant Human Ovarian Carcinoma Cells Display Nuclear Texture Changes Associated with Histone H4 Acetylation Morphometric Measurement of Prostate Structures as a Measure of Genomic Variability |
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Informatics Principles for Coding Data Analysis Methods Flow Cytometry Markup Language: A Proposal for Structured Representation of Experimental Metadata Cytometric Data Processing: From Modular Arrangement of Several Software Programs to an Integrative Database-Analysis System A New Data Mining/Knowledge Discovery Method for Flow Cytometry Defining a Bioinformatics System for FCS Data Files BioScope: Cytometry and the Internet for K-12 Education |
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Phytoplankton in the Aquatic Environment Analysis of Phytoplankton Communities in Lakes and Artificial Systems by Flow Cytometry and Laser Scanning Cytometry A Flow Cytometry Approach to Assess Oceanic Microbial Respiration In Situ On-line Analysis of Phytoplankton with CytoBuoy Monitoring Changes in Phytoplankton Populations in Ocean Samples using Artificial Neural Network Analysis of Flow Cytometry Data Oceanic Picoplankton: Large Scale Trends and Small Scale Variability Determined by Flow Cytometry Phytoplankton Size Spectra Measured by Flow Cytometry and Imaging in Flow in the Gulf of Maine and Coastal Waters |
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5:15 7:00 |
PARALLEL SESSION II |
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Digital Imaging Immunophenotyping of Lymph Node by Laser Scanning Cytometry (LSC) Coherent Multiprobes and Quantitative Spectroscopic Multimode Microscopy for the Study of Simultaneous Intracellular Events Quantitative In Situ Hybridization Using Peptide Nucleic Acid Probes: Methodological Aspects and Applications in Telomere Biology Automatic Quantification of Tumor Angiogenesis: Comparison of Two Staining Procedures and Three Color Characterization Methods Applications of Multispectral Imaging in Pathology and Cytology Spectral Pathology (SPY) - A Novel Method for Histo- and Cytological Analysis Data Annotation: The Bridge From Design to Interpretation A High Resolution Color Scanning Digital Microscope: A New Tool for Telepathy |
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Nano/Micro-Particle Technology/Rare Cell Detection Solubilization and Display of Seven Transmembrane Receptors on Beads for Real-time Fluorescence and Flow Cytometric Analysis Multiplex Analysis of Mutations in the RET-gene Using Suspension Arrays Novel Method for Multiplex High-throughput Flow Cytometric Analysis Simultaneous Quantitation of Six Human Cytokines by Microparticle Flow Cytometry Flow Cytometry with Excitation into Surface Plasmon Resonance Bands of Antibody-linker Gold or Silver Nanoparticle-aminodextran-polystyrene Bead Conjugates as White Blood Cell Markers The Role of Genetic Variation in Cervical Cancer Rare Event Detection in Human Genetic Biomonitoring: Enumeration and Sorting of Micronucleated Nascent Peripheral Blood Reticulocytes by Immunomagnetic Separation and Flow Cytometry Detection of Circulating Tumor Cells in Peripheral Blood Using Immunomagnetic Enrichment and Automated Cellular Imaging |
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Hematopoiesis/Stem Cell Biology Flow Cytometric High Throughput Screening for Optimizing Self Renewal of Primitive Human Hematopoietic Cells Data Pattern Analysis of Flow Cytometric Blood Leukocyte Immunophenotypes of Donors and Bone Marrow Transplant Recipients Clinical Interest of a Combination of Two Standardized Flow Cytometry Assays for Paroxysmal Nocturnal Hemoglobinuria Diagnosis Comparison of Two Single Platform Methodologies with Either Nucleic Acid Dye or Viability Dye for Enumeration of Hematopoietic Progenitor Cells in Blood-derived Specimens
Colony Formation by Flow Sorted Hematopoietic Stem Cells from C57Bl Mice: Interactions with Growth Regulatory Oligopeptides Fas/CD95 Expression Level After Stem Cell Transplantation: A Simple Parameter to Measure |
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Microbiology Multiparameter Flow Cytometry: Assessment of Bacterial Physiological State and the Application to the Study of the Scale-up of Bacterial Fermentations Glucose Uptake Dynamics in Single Cells Application of Flow Cytometry Using Fluorescent Probes to Study Substrate and Product Toxicity in the Indene Bioconversion |
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Monday, May 22, 2000 |
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7:30 5:30 |
Registration |
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8:00 9:45 |
TECHNICAL PLENARY I on Microarray and Chip Technology Fundamentals of Oligonucleotide Hybridizations PAM, A Flow Through Approach to Microarrays Microarrays for Analysis of Single Nucleotide Polymorphisms |
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9:45 10:45 |
Coffee Break/Exhibits |
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10:45 12:30 |
PARALLEL SESSION III |
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Microarrays I An Expression Microarray for Investigation of Genes Involved in Loss of Heterozygosity on Chromosome 16 in Breast Cancer Analyzing Gene Expression Data Array-based CGH for the Differential Diagnosis of Renal Cell Carcinoma Employing DNA Microarrays for the Analysis of Plant Gene Expression Technical Approaches for Producing and Analyzing DNA Microarrays Use of DNA Microarrays and Functional Proteomics in Drug Discovery |
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Clinical Immunology Simplified Quantitation of Myeloid Dendritic Cells in Peripheral Blood Using Flow Cytometry Denis Snider; Hong Liang; John Upham Quantitative Assessment of T Cell Function Using Integrated Calibration and Software Package and a 1:1 CD69:PE Reagent: Implications for Clinical Use to Document Immune Recovery Following Bone Marrow Transplantation Preoperative Prediction of Post-cardiotomy Syndrome (PCS) in Children Cardio-Pulmonary Bypass Surgery: Use of Cytometric Humor Parameters Neutrophil CD64 Expression in Acute Inflammatory Arthirides and in Systemic Infections Measurement of Error of Absolute CD4+ T Lymphocyte Count Double Platform Evaluations for the Assessment of Laboratory Performance in Lymphocyte Immunophenotyping Quality Control (QC) Ceramide Binds to and Signals Through the LPS Receptor CD14 that is Upregulated in Patients with Myocardial Infarction Flow Cytometry Evaluation of Peripheral Blood Leukocyte Function in Silicotic Patients Quantitation of Antigen-specific T-cells in Patients with Cancer Using VB-specific Antibodies |
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Cell Growth and Differentiation Multiparameter Flow Cytometry of the Cell Cycle Phosphorylated Histone H3 as a Marker of Mitosis and the Monocytic Phenotype Flow Cytometric Characterization of Hyperproliferation-associated Keratinization in Psoriatic Skin Different Expression Profiles of Human Cyclin B1 in Normal PHA-stimulated T Lymphocytes and Leukemic T Cells Combined Analysis of Ki-67, MCM3 and p27 Protein: a Snapshot of Cell Proliferation and Differentiation |
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Molecular and Clinical Genetics Isolation of Fetal Nucleated Red Blood Cells for Non-invasive Genetic Analysis Chromosomes Participating in Translocations Typical of Malignant Haemoblastoses are Also in Frequent Interchanges Induced by Densely Ionizing Radiation Features of Chromosomes in Human Terminally Differentiated Bronchial Cells Provide a Blueprint for Nuclear Organization Dynamics of DNA Repair in Living Cells A New Application for the Laser-scanning Cytometry: Fast Detection of Aneuploidy Induction in Sperm of Mice and Humans Identification of Genetic Markers for Prostate Cancer Progression |
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2:00 4:00 |
WORKSHOPS A |
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4:00 6:00 |
PARALLEL SESSION IV |
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Microarrays II Compact Disk Player Based Fluorescent Reader of Magnetically Aligned Objects in an Array Fluorocytometric Array System for Blood Group Serology, Nucleic Acid Testing, and Immunoassays Performed by Blood Banks High Throughput Molecular and Genomic Analyses Using Microspheres Multiplex Analysis of Mutations in the RET-gene Using Suspension Arrays Multiplexed Single Nucleotide Polymorphism Analysis by Flow Cytometry Kimberly Slentz-Kesler; Michael P. Weiner Oligonucleotide-array-based Comparative Genomic Hybridization Optical Transduction of Structural Changes of Proteins Using Surface Enhanced Nano Cluster Resonance |
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Cytometry for the Industrial Laboratory Application of Flow Cytometry to Bioprocess Development, Validation and Control Flow Cytometry as a Tool for Pharmaceutical Assay Development Flow Cytometry Approaches to Assess Stress and Food Preservation Effects on Bacterial Physiology and Survival Sorting, In Vitro Differentiation and Phenotyping of Mouse and Human Osteoclast Precursors Flow Cytometric Cell Sorting - An Indispensable Tool for Drug Discovery Lisa Green; Fred Chadwell; Mary Beth Anania; Larry Mann; Jonathan Lee; Philip Marder Cell Encapsulation and Flow Cytometry. Incorporation Into Cell Line Development Programs Comparison of Transient Transfection Efficiency Using Commercially Available Transfection Reagents Murine Bone Marrow Toxicity Assessment in Pre-clinical Drug Development by Flow Cytometry |
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Cell Death I Square Wave Pulse Electropermeabilization of Cytochrome C Induced Apoptosis Prooxidant and Antioxidant Effect of Melatonin on Human Liver Cell Line: Assessment Using Microplate Cold Light Cytofluorimetry (MCCM) Directly on Live Adherent cells Dynamics of Growth and Death in Antigen Specific Swine T-cell Subset Proliferation In Vitro New Approaches in Analysis of Cell Death by Laser Scanning Cytometry Monitoring Cell Death by Laser Scanning Cytometry Individual Radiosensitivity: Search for Genetic Markers Continuous Monitoring and Analysis of Apoptotic Process in Individual Cells |
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Basic Immunology Sensitive Detection of APC Subpopulations According to the Presentation of a Specific Antigen Identification of a Novel CD56+ Non-cytotoxic NK Cell Subset Identification of a Human Recent Thymic Emigrant Phenotype Confocal Analysis of the Nuclear Translocation NFkB Family Members During the Maturation of Monocyte Derived Dendritic Cells Normal Human B-cell Differentiation: Quantitative Analysis of Levels of Bcl-2 Expression Identification of Live Antigen-reactive T Cells by Flow Cytometry |
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6:00 pm 8:00 pm |
Commercial Tutorials/Exhibits/Happy Hour |
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8:00 |
Dinner on your own |
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Tuesday, May 23, 2000 |
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8:00 9:45 |
Technical Plenary II on Microarray and Chip Technology Analysis of DNA Copy Number Variations Using Array CGH Analysis, Interpretation and Evaluation of Gene Expression Data |
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9:45 10:45 |
Coffee Break/Exhibits |
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10:45 12:30 |
Symposium on In Vivo Molecular and Cellular Imaging Towards In Vivo Molecular Imaging Optical Bioimaging |
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12:30 2:00 |
Lunch/Exhibits/Posters |
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2:00 4:00 |
WORKSHOPS B |
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6:00 8:00 |
Commercial Tutorials/Exhibits/Happy Hour |
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Dinner on your own |
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Wednesday, May 24, 2000 |
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7:30 5:30 |
Registration |
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7:45 9:45 |
Biology Plenary Session on Cell Cycle Regulation and Analysis 4D Control of Mitosis The Activity and Expression of Cyclin D1 in Individual Cycling Cells Cell Cycle Kinetics and Transient Transfection Measurement of Kinetic Variability Using Three Color Flow Cytometry Ultraviolet-induced Detection of Halogenated Pyrimidines in DNA Replicating Cells |
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9:45 10:45 |
Coffee Break/Exhibits |
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10:45 12:30 |
New Investigator/Student Symposium |
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12:30 2:00 |
Lunch/Exhibits/Posters |
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2:00 4:00 |
Workshops C |
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4:00 6:00 |
PARALLEL SESSION V |
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Image Cytometry: Technical Advances Direct Neural Network Application for Automated Cell Recognition Glass Slide Scanning: A Fast and Inexpensive Answer to the Problem of Tumor Tissue Heterogeneity for Quantimetry Fuzzy Classification of Color Images: Application to Quantitative Immunohistochemistry Advances in High Resolution Cytometry of FISH Dots in Interphase Cell Nuclei Segmentation of Cell Nuclei using Immunolabeled Nuclear Membrane Proteins
Flow Cytometry Aids Histology in Identifying Low and High Risk Subsets of Patients in Barretts Esophagus Towards a Model for Neoplastic Progression in Barretts Esophagus Genetic Analysis of Dysplasias and Cancers of the Uterine Cervix Using CGF, FISH and RT-PCR Proliferative Potential of Neuroblastoma Cells Disseminated in the Hematopoietic System Developing a Unified Prognostic Model for Node Negative Breast Cancer Patients From Flow Cytometric DNA Histograms Adenocarcinoma Ccell Phenotyping by Flow Cytometry Has Tpot Gone to Pot?
Simultaneous Immunofluorescence and In Situ PCR for Determination of HIV Viral Load in Immune Cell Subsets Redox, Activation and Apoptotic Status of Monocytes From HIV-infected Patients The Use of In Cell Viral Load (ViroTect™) as a Tool to Study Infected Subpopulatoins Seen in HIV-infected Individuals Single Platform T-cell Enumeration Involving the Multiple Sites of the Canadian Quality Assessment Program for Immunophenotyping Evaluation of the Flow Cytometric Quantitation of CD95 (APO-1/FAS) as an Alternative to CD38 Quantitation in HIV+ Young Children Flow Cytometric Detection of the Inhibition of Virus Entry and Replication Using a Broadly Reactive Antibody and Subtype A-G Clinical Isolates of HIV-1 Kovit Pattanapanyasat HHV-6 Induction of HIV Receptors and Co-receptors on Human Hematopoietic Stem/ Progenitor Cells |
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Molecular Pharmacology and Cellular Physiology Determination of the Michaelis-Menton Constant (Km) of Intracellular Enzymatic Reaction for Individual Live Lymphocytes Histidylated Oligolysines Increase Transmembrane Passage and the Biological Activity of Antisense Oligonucleotides Flow Cytometric Quantitation of Integrin Expression on Cultured Fibroblasts or Keratinocytes for the Study of Cosmetic Active Ingredients |
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Thursday, May 25, 2000 |
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7:30 5:30 |
Registration |
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7:45 9:45 |
Clinical Plenary on Hematology Cytogenetically Aberrant Cells in Stem Populations in Hematologic Malignancies Immunophenotyping of Heamatological Malignancies: Conventional and New Clinical Applications Antitumor Strategies Based on Modulation of NFkB Pathway: Enhancement of Cell Propensity to Apoptosis by Onconase Dynamic Fluorescence Imaging: T Cell Activation |
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9:45 10:45 |
Coffee Break/Exhibits |
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10:45 12:30 |
PARALLEL SESSION VI Enzymatic Amplification Staining for Flow Cytometric Analysis of Cell Surface Molecules A Compact Solid-state 488 nm Laser for Cell Analysis Applications Development of a Platform Independent, Public Domain FCS File Reading and Writing Package A Method for Differentiating Between Diverse Fluorophores of Similar Spectra Based on the Measurement of Their Fluorescence Polarization Routine 6-color Flow Cytometry Using a 9 Parameter, 3 Laser Bench Top Analyzer How To Do Four Color Compensation Digital Data Acquisition System for Flow Cytometric Analysis A Simulation of Chromosome Sorter Using Self-organizing Map |
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Cell Death II Caspase 3 Activation as a Marker of Pre-apoptotic Leukemic Cells Apoptotic Cell Death: Correlated Cellular Changes in Multiple Apoptotic Signals in Individual Cells Analysis of Laser Scattering Pattern as an Early Measure of Apoptosis A Multiparameter Flow Cytometric Analysis of the Action of Melittin, a Bee Venom Toxin on Nucleated Mammalian Cells TNA-alpha and Interferon Gamma Pathways to Apoptosis in Human Tumor Cell Programmed Cell Death Specific to the Unicellular Life of Dictyostelium Discoideum Relies on Apoptosis |
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Hematological Malignancy Minimal Residual Disease Investigation in Multiple Myeloma by Immunophenotypical Analysis Genotypes and Phenotypes in B-cell Non-Hodgkins Lymphoma Flow Cytometric Detection of Clonality in Mature T Cell Malignancies by Use of a Novel T Cell Receptor Vbeta Antibody Kit Clinical Significance of the Apoptotic Proteins in Acute Myeloid Leukemia (AML) Immunophenotype Characterization of Myelodysplastic Syndromes: Results of a Multicentric Analysis by Flow Cytometry Absence of Detectable Ph+ Cells in CD34- LIN- "SP" Bone Marrow Progenitor Cells From Patients With Chronic Myeloid Leukemia (CML) |
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Aquatic Bacteria in the Microenvironment Flow and Image Cytometric Analysis of Protozoan Grazing Pressure on a Bacterial Community in a Two-stage Flow-through System (chemostat) Mediterranean Coastal Water Eutrophication and Patterns of Microorganism Abundances are Estimated From Flow Cytometric Measurements Picophytoplankton Ingestion by the Appendicularia Oikopleura Dioica in Natural and Artificial Environment Applications of Laser Scanning, Flow and Epifluorescence Cytometry to the Rapid Detection of Bacteria and Viability Assessment Cytometry and Environmental Microbiology Detection of Functional Subgroups in Heterotrophic Bascterioplankton by Flow Cytometry |
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12:30 2:00 |
Lunch/Exhibits/Posters |
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2:00 4:00 |
Workshops D |
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5:30 6:30 |
Robert Hooke Distinguished Lecture As at the end of the nineteenth century, science is at the point when conceptual advances are needed further to deepen the understanding of the world. Here are some of the issues to be dealt with:
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6:30 7:30 |
Awards |
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7:30 10:30 |
Final Night Event |