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An Introduction to Molecular Biotechnology: Fundamentals, Methods and Applications, 3rd Edition

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An Introduction to Molecular Biotechnology: Fundamentals, Methods and Applications, 3rd Edition

Michael Wink

ISBN: 978-3-527-34414-7 January 2020 700 Pages

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Description

Completely updated in line with the rapid progress made in the field, this new edition of the highly-praised textbook addresses powerful new methods and concepts in biotechnology, such as genome editing, reprogrammed stem cells, and personalized medicine.
An introduction to the fundamentals in molecular and cell biology is followed by a description of standard techniques, including purification and analysis of biomolecules, cloning techniques, gene expression systems, genome editing methods, labeling of proteins and in situ-techniques, standard and high resolution microscopy. The third part focuses on key areas in research and application, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies and systems biology. The final part looks at the biotechnology industry, explaining intellectual property issues, legal frameworks for pharmaceutical products and the interplay between start-up and larger companies. The contents are beautifully illustrated throughout, with hundreds of full color diagrams and photographs.
Provides students and professionals in life sciences, pharmacy and biochemistry with everything they need to know about molecular biotechnology.
FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY
The Cell as the Basic Unit of Life
Structure and Function of Cellular Macromolecules
Structure and Functions of a Cell
Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins)
Distributing Proteins in the Cell (Protein Sorting)
Evolution and Diversity of Organisms

STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY
Isolation and Purification of Proteins
Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry
Isolation of DNA and RNA
Chromatography and Electrophoresis of Nucleic Acids
Hybridization of Nucleic Acids
Use of Enzymes in the Modification of Nucleic Acids
Polymerase Chain Reaction
DNA Sequencing
Genome Editing
Cloning Procedures
Expression of Recombinant Proteins
Omics technologies
Patch Clamp Method
Cell Cycle Analysis
Microscopic Techniques
Laser Applications

KEY TOPICS
Genomics and Functional Genomics
Bioinformatics
Cellular Systems Biology
Protein-Protein and Protein-DNA Interaction
Drug Research
Drug Targeting and Prodrugs
Molecular Diagnostics and Personalized Medicine
Recombinant Antibodies and Phage Display
Transgenic and Gene-Targeted Mice
Plant Biotechnology
Industrial Biocatalysis

BIOTECHNOLOGY IN INDUSTRY
The Biotech Industry, Markets, and Opportunities
Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues
Drug Approval in the European Union and United States
Founding a Biotech Company

APPENDIX
Further Reading
Abbreviations
Subject Index