Chapter 1: Introduction (Pengcheng Fu).
Chapter 2: Basics of Molecular Biology, Genetic Engineering and Metabolic Engineering (Michael X. Wang and Huidong Shi).
Chapter 3: High-Throughput Technologies and Functional Genomics (Henry Wang and Dorothea K. Thompson).
Chapter 4: Predicting the Lymph Node Status of Breast Cancer Tumors Using Gene Expression Patterns and Genomic Signal Processing (Gordon Okimoto).
Chapter 5: From Recombinant Genes to Recombinant Genomes for Systems Biology (Mitsuhiro Itaya).
Chapter 6: In silico Genome-scale Metabolic Models: The Constraint-based Approach and its Applications (Andrew Joyce and Bernhard Palsson).
Chapter 7: Mathematical Modeling of Genetic Regulatory Networks: Stress Responses in Escherichia coli (Delphine Ropers, Hidde de Jong, and Johannes Geiselmann).
Chapter 8: Systems Approaches in Molecular Diagnostics and Drug Development (Martin Latterich).
Chapter 9: Yeast as a Prototype for Systems Biology (Gautham Vemuri and Jens Nielsen).
Chapter 10: Construction and Applications of Genome-Scale In Silico Metabolic Models for Strain Improvement (Sang Yup Lee, Jin Sik Kim, Hongseok Yun, Tae Yong Kim, Seung Bum Sohn, Hyun Uk Kim).
Chapter 11: Synthetic Biology: Putting Engineering into Bioengineering (Matthias Heinemann and Sven Panke).
Chapter 12: Rationals of Gene Design and de novo Gene Construction (Marcus Graf, Thomas Schoedl and Ralf Wagner).
Chapter 13: Self-replication in Chemistry and Biology (Phil Holliger and David Loakes).
Chapter 14: The Synthetic Approach for Regulatory and Metabolic Circuits (Wilson W. Wong and James C. Liao).
Chapter 15: Synthetic Genetic Networks (David Greber and Martin Fussenegger).
Chapter 16: The theory of biological robustness and its implication to cancer (Hiroaki Kitano).
Chapter 17: Nucleic Acid Engineering (Wenlong Cheng, Liang Ding, Hisakage Funabashi, Nokyoung Park, Soong Ho Um, Jianfeng Xu, and Dan Luo).
Chapter 18: Potential Applications of Synthetic Biology in Marine Microbial Functional Ecology and Biotechnology (Guangyi Wang and Juanita Mathews).
Chapter 19: On fundamental implications of systems and synthetic biology (Cliff Hooker).
Chapter 20: Outstanding Issues in systems and synthetic biology (Pengcheng Fu and Cliff Hooker).