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The HPLC Expert: Possibilities and Limitations of Modern High Performance Liquid Chromatography




The HPLC Expert: Possibilities and Limitations of Modern High Performance Liquid Chromatography

Stavros Kromidas (Editor)

ISBN: 978-3-527-67763-4 April 2016 357 Pages



The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.
Survey of current LC/MS instruments
Pitfalls of LC/MS coupling
Ion chromatography as an application example for LC/MS
Separation of biomolecules by chromatographic techniques
Gradient optimization for RP-HPLC
Gradient prediction
Optimization strategies for RP-HPLC
Integration methods and strategies
HPLC software: performance, comparison, developments
Intelligent documentation of HPLC data for regulated studies
How to survive an FDA inspection
Mining the web for HPLC data
Selecting and comparing HPLC columns
HPLC hardware: recent trends

"This book offers a unique perspective on HPLC, with a unique selection of topics that are becoming of even greater importance in the rapidly expanding field of this technique. The review articles, contributed by well-qualified experts in their fields, provide a comprehensive overview of the hottest tasks in the field of modern HPLC, and represent a valuable source for specialists in this area of separation science...Interested readers will find quick and reliable guidance to overcome some common mistakes and drawbacks in the practice of HPLC, as well as useful tips and links for efficient information collection." (Analytical and Bioanalytical Chemistry 23/05/2017)