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HPLC and UHPLC for Practicing Scientists, 2nd Edition




HPLC and UHPLC for Practicing Scientists, 2nd Edition

Michael W. Dong

ISBN: 978-1-119-31377-9 July 2019 416 Pages



A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries

Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. 

HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. 

  • Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references
  • Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics
  • Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC
  • Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects
  • Includes end-of-chapter quizzes as assessment and learning aids
  • Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries

Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.

About the author

Biographies of Contributors



Chapter 1: Introduction

Chapter 2: Basic Terms and Concepts

Chapter 3: HPLC Columns and Trends

Chapter 4: HPLC/UHPLC Instrumentation and Trends

Chapter 5: UHPLC: Perspectives, Performance, Practices, and Potential Issues

Chapter 6: LC/MS: Fundamentals, Perspectives, and Applications

Chapter 7: HPLC/UHPLC Operation Guide

Chapter 8: HPLC/UHPLC Maintenance and Troubleshooting

Chapter 9: Pharmaceutical Analysis

Chapter 10: HPLC method development

Chapter 11: Regulations, HPLC System Qualification, Method Validation, and Transfer

Chapter 12: HPLC and UHPLC for Biopharmaceutical analysis

Chapter 13: HPLC Applications in food, Environmental, Chemical, and Life sciences analysis