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Analysing Gene Expression: A Handbook of Methods, Possibilities, and Pitfalls

Analysing Gene Expression: A Handbook of Methods, Possibilities, and Pitfalls

Stefan Lorkowski (Editor), Paul M. Cullen (Editor)

ISBN: 978-3-527-60533-0

Mar 2006

992 pages

Select type: E-Book



This book combines the experience of 225 experts on 900 pages.

Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.




Table of Contents.

List of Contributors.

1. Basic concepts of gene expression.

2. Sample preparation and supplementary tools.

3. Methods for analysing mRNA expression.


4. High-throughput and industrial methods for mRNA expression analysis.

5. Protein expression analysis.

6. Methods for mRNA and protein expression analysis in situ and in vivo.

7. Computational methods and bioinformatic tools.


""Analysing Gene Expression bietet zuverlässige Information für Neueinsteiger, aber auch für Profis und ist auf jeden Fall ein Gewinn!""
Angewandte Chemie

""Es liegt hier insgesamt ein empfehlenswertes, sehr umfassendes Handbuch vor, das eine äußerst bemerkenswerte, kritische Zusammenstellung wichtiger Methoden liefert, und nicht zuletzt durch die sehr gründlichen Referenzlisten besticht. Gemessen am üppigen Informationsgehalt erscheint dieses für die molekularbiologische Handbibliothek obligatorische Werk zum Thema Genexpression preislich angemessen.""
R. Einspanier
European Food Research and Technology