DescriptionThis is a survey of well characterized and recently discovered bacterial protein toxins. Leading investigators of the respective toxins review the various molecular mechanisms of action, ranging from toxin-induced ADP-ribosylation up to membrane perforation by pore-forming toxins. Thy also describe the consequences on host physiology before focusing on potential applications as cell biological and pharmacological tools for research and medical applications. Detailed descriptions of the methodology include the engineering and use of modified and chimeric toxins for better performance.
A solid introduction to toxin structure and functions, as well as a valuable source of methodology for researchers in molecular biology, pharmacology and experimental medicine.
Chapter 2: Cholera Toxin and Escherichia coli Heat-labile Enterotoxin: Biochemical Methods for Assessing Enzymatic Activities (W. Patton, et al.).
Chapter 3: Pertussis Toxin (C. Locht and R. Antoine).
Chapter 4: Pertussis Toxin as a Cell Biology Tool (B. Nürnberg).
Chapter 5: Clostridium botulinum ADP-ribosyltransferase C3 (K. Aktories and G. Koch).
Chapter 6: Clostridium botulinum C3 Exoenzyme and Studies on Rho Proteins (C. Nobes and A. Hall).
Chapter 7: Preparation of Clostridium botulinum C3 Exoenzyme and Application of ADP-ribosylation of Rho Proteins in Biological Systems (Y. Saito and S. Narumiya).
Chapter 8: Actin-ADP-ribosylating Toxins: Cytotoxic Mechanisms of Clostridium botulinum C2 Toxin and Clostridium perfringens lota Toxin (K. Aktories, et al.).
Chapter 9: Purification, Activation and Endocytosis of Botulinum C2 Toxin (I. Ohishi).
Chapter 10: The Role of the Clostridium botulinum C2 Toxin as a Research Tool of Study Eucaryotic Cell Biology (I. L. Simpson).
Chapter 11: Probing the Actin Cytoskeleton by Clostridium botulinum C2 Toxin and Clostridium perfringes lota Toxin (K. Aktories, et al.).
Chapter 12: Clostridium difficile Toxins (M. Thelestam, et al.).
Chapter 13: Clostridium difficile Toxin B as a Probe for Rho GTPases (I. Just, et al.).
Chapter 14: Clostridial Neurotoxins (G. Schiavo and C. Montecucco).
Chapter 15: Isolated Nerve Terminals as a Model System for the Study of Botulinum and Tetanus Toxins (J. Blasi, et al.).
Chapter 16: Clostridial Toxins and Endocrine Secretion: Their Use in Insulin-Secreting Cells (J. Lang, et al.).
Chapter 17: Pore-forming Toxins (S. Bhakdi, et al.).
Chapter 18: Application of Alpha-toxin and Streptolysin O as Tools in Cell Biological (G. Ahnert-Hilger and U. Weller).
Chapter 19: Toxins as Transporting Tools (S. Olsnes, et al.).
Chapter 20: A Brief Guide to the Safe Handling of Biological Toxin (A. B. Maksymowych and L. L. Simpson).