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Biocatalysts and Enzyme Technology, 2nd Edition

Biocatalysts and Enzyme Technology, 2nd Edition

Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer

ISBN: 978-3-527-32989-2 December 2012 Wiley-Blackwell 626 Pages


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This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology.
The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added.
Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery,
characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell
systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the
sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered.

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Introduction to enzyme technology

Basics of enzymes as biocatalysts

Enzyme discovery and protein engineering

Enzymes in organic chemistry

Cells designed by metabolic engineering as biocatalysts for multi-enzyme biotransformations

Enzyme production and purification

Application of enzymes in solution: Soluble enzymes and enzyme systems

Immobilization of enzymes (Including Applications)

Immobilization of microorganisms and cells

Characterization of immobilized biocatalysts

Reactors and process technology

Case study: The one-step enzymatic process to produce 7-ACA from cephalosporin C

Appendix I
The World of Biotechnology Information: Seven Points for Reflecting on Your Information Behavior

Appendix II
Solutions to exercises

Appendix III
Symbols, additional Symbols