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Cell Culture Engineering: Recombinant Protein Production

Cell Culture Engineering: Recombinant Protein Production

Gyun Min Lee, Helene Faustrup Kildegaard, Sang Yup Lee, Jens Nielsen, Gregory Stephanopoulos

ISBN: 978-3-527-34334-8

Feb 2019

400 pages

Select type: Hardcover

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Part of the "Advanced Biotechnology" series, this volume covers all important aspects of cell culture engineering as applied to the production of recombinant proteins for therapeutic use, from molecular biology to bioprocess engineering.
With a focus on mammalian and animal cells, the book describes the challenges in cell line development and cell engineering, while providing an overview of synthetic and systems biology approaches applied to cell culture engineering. In addition, the authors elaborate the use of CHO cells as common cell line for protein production and discuss current perspectives in production processes, including cell culture media, batch, fed-batch and perfusion processes, as well as process analytical technology, quality by design and scale down models.
1. A platform technology for therapeutic protein production
2. Cell line development for therapeutic protein production
3. Development of a transient gene expression upstream platform
4. CHO genome: Enhancing product and bioprocess attributes using genome-scale models of CHO metabolism
5. Epigenetics and stability: An investigation on genome heterogeneity and stability and on what controls cell behavior
6. CHO synthetic biology
7. Omics data and mammalian systems biotechnology: an integrative framework for combining in silico modeling and multi-omics datasets in different CHO parental cell lines
8. Mammalian gene editing tools
9. Cell Engineering
10. Metabolite Profiling of Mammalian Cells
11. Application of 13C flux analysis for cell engineering
12. Host cell protein impurity
13. Batch and fed-batch culture
14. Perfusion culture
15. Process analytical technology and quality-by-design for animal cell culture
16. Development of a scale down cell culture model using multivariate analysis as a qualification tool