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Chromatography: Concepts and Contrasts, 2nd Edition

Chromatography: Concepts and Contrasts, 2nd Edition

James M. Miller

ISBN: 978-0-471-98059-9

Dec 2005

520 pages



The first edition of Chromatography: Concepts and Contrasts, published in 1988, was one of the first books to discuss all the different types of chromatography under one cover. The second edition continues with these principles but has been updated to include new chapters on sampling and sample preparation, capillary electrophoresis and capillary electrochromatography (CEC), chromatography with mass spec detection, and industrial and governmental practices in regulated industries.
  • Covers extraction, solid phase extraction (SPE), and solid phase microextraction (SPME), and introduces mass spectrometry
  • Updated with the latest techniques in chromatography
  • Discusses both liquid chromatography (LC)and gas chromatography(GC)
Preface to Second Edition.


Preface to the First Edition.

Symbols, Abbreviations, and Acronyms.

1 Impact of Industrial and Government Regulatory Practices on Analytical Chromatography.

1.1 Locus of Chromatography in Chemical Industry.

1.2 Governmental Organizations.

1.3 Nongovernmental Agencies.

1.4 Standards, Calibration, and NIST.

1.5 USP and Other Pharmacopoeias.

1.6 International Guidelines for Analytical Laboratories.

1.7 Final Comments.


2 Introduction to Chromatography.

2.1 Brief History.

2.2 Definitions and Classifications.

2.3 Summary.


3 Band Broadening and Kinetics.

3.1 Configurations of the Stationary Phase.

3.2 Rate Theory.


4 Physical Forces and Interactions.

4.1 Intermolecular and Interionic Forces.

4.2 Size Exclusion—Molecular Sieving.

4.3 Some Models.


5 Optimization and the Achievement of Separation.

5.1 Kinetics and Zone Broadening.

5.2 Thermodynamics and Zone Migration.

5.2 Achievement of Separation.

5.4 Optimization of Separations.


6 Comparisons Between Chromatographic Modes.

6.1 Gas Chromatography Compared to Liquid Chromatography.

6.2 Supercritical Fluids and Supercritical Fluid Chromatography.

6.3 Reduced Parameters.

6.4 Columnar and Planar Configurations.


7 Gas Chromatography.

7.1 Early History, Theories, and Classifications.

7.2 Instrumentation for Capillary GC.

7.3 Instrumentation for Packed-Column GC.

7.4 Stationary Phase.

7.5 Temperature Effects.

7.6 Special Topics.

7.7 Summary and Evaluation.


8 Liquid Chromatography in Columns.

8.1 Introductory Classifications.

8.2 Classification of HPLC Modes.

8.3 Instrumentation.

8.4 Reversed-Phase Method Development and Optimization.

8.5 RP-HPLC Alternatives for the Pharmaceutical Industry.

8.6 Preparative LC.

8.7 Special Topics.

8.8 Summary and Evaluation.


9 Quantitation: Detectors and Methods.

9.1 Detectors.

9.2 Data Acquisition and Processing.

9.3 Quantitative Analysis.


10 Chromatography with Mass Spectral Detection (GC/MS and LC/MS).

10.1 Basics of Mass Spectrometry.

10.2 Gas ChromatographyrMass Spectrometry.

10.3 Liquid ChromatographyrMass Spectrometry.

10.4 Other Hyphenated Methods.

10.5 Summary.


11 Liquid Chromatography on Plane Surfaces.

11.1 Paper Chromatography.

11.2 Thin-Layer Chromatography.

11.3 Other Topics.

11.4 Literature Summary and Applications.


12 Qualitative Analysis.

12.1 Retention Parameters.

12.2 Other Methods of Qualitative Analysis.


13 Capillary Electrophoresis and Capillary Electrochromatography 365

13.1 Principles of Electrophoresis.

13.2 Zone Electrophoresis.

13.3 Capillary Electrophoresis.

13.4 Capillary Electrochromatography.


14 Sample Preparation.

14.1 Extraction.

14.2 Dialysis.

14.3 Derivatization.


15 Special Applications.

15.1 Multidimensional Chromatography.

15.2 Biological Applications.

15.3 Chiral Separations.

15.4 Other Topics.


16 Selection of a Method.

16.1 Methods of Attack.

16.2 The Internet.

16.3 Experimental Approach.

16.4 Summary.


Some Internet Web Sites of Interest to Chromatographers.

Appendix A ICH Glossary.

Appendix B.


New chapters on sampling and sample preparation; capillary electrophoresis and capillary electrochromatography (CEC), chromatography with mass spec detection; and industrial and governmental practices in regulated industries.
"Of course, I found a few mistakes, but not enough to criticize what is a useful, and, if not unique, at least a satisfying alternative approach to introducing a broad audience to the science, practice, and purpose of chromatography in a global context." (Chromatographia, August 2010)

…a practical, hands-on approach…" (CHOICE, July 2005)

"…an excellent introduction to all the chromatographic techniques for both the industrial practitioner and the…student because of its unified treatment, its modern approach, and up-to-date references." (Journal of the American Chemical Society, June 29, 2005)

"Professor Miller should be congratulated for the book...should be on the shelf of every practicing chromatographer...students taking separations science courses could also use it as their standard textbook." (Chromatographia, February 2005)

  • Contains new chapters on applications such as sampling and sample preparations; capillary electrophoresis and capillary electrochromatography (CEC); chormatography with mass spec detection
  • Includes a new chapter on industrial and governmental practices that introduces practices in regulated industries such as the pharmaceutical industry, which is regulated by the FDA
  • Provides a unified treatment of the various chromatographic techniques
  • Offers a readable, moderate-level text and reference for chromatography
  • Discusses both liquid chromatography (LC) and gas chromatography (GC)